Phusion
From OpenWetWare
(Difference between revisions)
| Line 13: | Line 13: | ||
*[http://www.neb.com/nebecomm/products/productF-530.asp Purchase] | *[http://www.neb.com/nebecomm/products/productF-530.asp Purchase] | ||
*[http://www.neb.com/nebecomm/ManualFiles/manualF-540.pdf Manual] | *[http://www.neb.com/nebecomm/ManualFiles/manualF-540.pdf Manual] | ||
| + | |||
| + | [[Category:Material]] [[Category:Enzyme]] [[Category:Polymerase]] [[Category:DNA]] | ||
Revision as of 10:22, 24 May 2007
Description
Fusion of a Pyrococcus (Pfu) -like enzyme with a double-strand DNA binding domain → increased processivity.
Statistics about Phusion™ High-Fidelity DNA Polymerase
- 10x processivity compared to Taq
- 50x fidelity compared to Taq
- Creates blunt end
- Error rate is 4.4 X 10-7 in Phusion HF buffer and 9.5 X 10-7 in GC buffer
- Non-displacing
