Phusion

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*[http://www.neb.com/nebecomm/products/productF-530.asp Purchase]
*[http://www.neb.com/nebecomm/products/productF-530.asp Purchase]
*[http://www.neb.com/nebecomm/ManualFiles/manualF-540.pdf Manual]
*[http://www.neb.com/nebecomm/ManualFiles/manualF-540.pdf Manual]
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[[Category:Material]] [[Category:Enzyme]] [[Category:Polymerase]] [[Category:DNA]]

Revision as of 09:22, 24 May 2007

Description

Fusion of a Pyrococcus (Pfu) -like enzyme with a double-strand DNA binding domain → increased processivity.

Statistics about Phusion™ High-Fidelity DNA Polymerase

  • 10x processivity compared to Taq
  • 50x fidelity compared to Taq
  • Creates blunt end
  • Error rate is 4.4 X 10-7 in Phusion HF buffer and 9.5 X 10-7 in GC buffer
  • Non-displacing

References

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