Phleomycin - Revision history

Reshma P. Shetty at 17:47, 26 July 2007

Reshma P. Shetty — Thu, 26 Jul 2007 17:47:49 GMT

←Older revision		Revision as of 17:47, 26 July 2007
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	The best price I've found is through Invivogen		The best price I've found is through Invivogen
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		+	[[Category:Material]] [[Category:Antibiotic]]

Jgritton at 13:55, 1 June 2005

Jgritton — Wed, 01 Jun 2005 13:55:34 GMT

←Older revision		Revision as of 13:55, 1 June 2005
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	Phleomycin is pH sensitive--it loses its effect at low pH. When making phleomycin plates add 2 pellets of NaOH per liter to get the pH above 7.		Phleomycin is pH sensitive--it loses its effect at low pH. When making phleomycin plates add 2 pellets of NaOH per liter to get the pH above 7.

-	The stock solution is 10 ug/mL (~~1000X~~), is light blue and is stored at -20 C. It should be stable for 1 year.	+	The stock solution is 20 ug/mL (2000X), is light blue and is stored at -20 C. It should be stable for 1 year.
		+
		+	The best price I've found is through Invivogen

Jgritton at 15:41, 12 May 2005

Jgritton — Thu, 12 May 2005 15:41:20 GMT

←Older revision		Revision as of 15:41, 12 May 2005
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-	~~Used to select for~~ the ble ~~marker in yeast.~~	+	==Mode of Action==
-		+	Binds and cleaves DNA
		+	==Mechanism of Resistance==
		+	The product of the ''Sh ble'' gene binds to phleomycin and inhibits its ability to cleave DNA
		+	==Usage==
	After transformation incubate culture in YPD at 30 C for 6 hours to overnight, then chill on ice for 1 hour. Plate on YPD (pH 7) containing 10 mg/L phleomycin.		After transformation incubate culture in YPD at 30 C for 6 hours to overnight, then chill on ice for 1 hour. Plate on YPD (pH 7) containing 10 mg/L phleomycin.

Jgritton at 17:38, 6 May 2005

Jgritton — Fri, 06 May 2005 17:38:42 GMT

New page

Used to select for the ble marker in yeast.

After transformation incubate culture in YPD at 30 C for 6 hours to overnight, then chill on ice for 1 hour. Plate on YPD (pH 7) containing 10 mg/L phleomycin.

Phleomycin is pH sensitive--it loses its effect at low pH. When making phleomycin plates add 2 pellets of NaOH per liter to get the pH above 7.

The stock solution is 10 ug/mL (1000X), is light blue and is stored at -20 C. It should be stable for 1 year.