Phleomycin: Difference between revisions
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Revision as of 10:38, 6 May 2005
Used to select for the ble marker in yeast.
After transformation incubate culture in YPD at 30 C for 6 hours to overnight, then chill on ice for 1 hour. Plate on YPD (pH 7) containing 10 mg/L phleomycin.
Phleomycin is pH sensitive--it loses its effect at low pH. When making phleomycin plates add 2 pellets of NaOH per liter to get the pH above 7.
The stock solution is 10 ug/mL (1000X), is light blue and is stored at -20 C. It should be stable for 1 year.
