Pfx50

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Contents

Description

Fusion of a Thermococcus zilligii polymerase with an accessory protein. Contains proofreading 3'->5' exonuclease activity. Accesory protein stabilizes primer-template complexes in PCR

Statistics about Pfx50 Polymerase

  • 50x fidelity compared to Taq
  • 15 second per kb, ideal 60 sec per kb

Quick reference reaction mix

See the manual for details and special usage conditions.

Component Volume for 50μl reaction Final concentration
10x Pfx50 PCR mix 5μl 1x
10mM dNTPs 1μl 200μM each
primer A x μl 0.5μM
primer B x μl 0.5μM
template DNA xμl
H2O add to 50μl
Pfx50 DNA polymerase (5U/μ) 0.5μl 0.02 U/pl

Thermocycling conditions

  1. 15-30 s/kb extension time.
  2. 98C for denaturation.
  3. Anneal at 2C lower than the Tm.
  4. Extend at 72C.

References

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