Pfu
From OpenWetWare
(Difference between revisions)
(→Procurement) |
|||
| Line 9: | Line 9: | ||
*Pfu has proof reading activity which makes it more accurate than for example Taq. | *Pfu has proof reading activity which makes it more accurate than for example Taq. | ||
*Pfu does not exhibit 5' -> 3' exonuclease activity, which prevents degradation of the primers in mutagenesis methods based on ligation of primers or elongated primers. | *Pfu does not exhibit 5' -> 3' exonuclease activity, which prevents degradation of the primers in mutagenesis methods based on ligation of primers or elongated primers. | ||
| + | |||
| + | |||
| + | ==Characteristics== | ||
| + | *Elogation speed (bp/sec) tends to be lower than for example Taq. | ||
| + | *Build-in "Hotstart", because it's only active at high temperature, unlike Taq which has some activity at lower temperatures too | ||
| + | *Can degrade oligonucleotides, causing smearing (on gel). | ||
[[Category:Material]][[Category:Enzyme]][[Category:Polymerase]][[Category:DNA]] | [[Category:Material]][[Category:Enzyme]][[Category:Polymerase]][[Category:DNA]] | ||
Revision as of 14:02, 23 April 2008
Contents |
Purpose
- Pfu is a thermostable DNA polymerase that can be used for PCR.
Procurement
Use
- Pfu has proof reading activity which makes it more accurate than for example Taq.
- Pfu does not exhibit 5' -> 3' exonuclease activity, which prevents degradation of the primers in mutagenesis methods based on ligation of primers or elongated primers.
Characteristics
- Elogation speed (bp/sec) tends to be lower than for example Taq.
- Build-in "Hotstart", because it's only active at high temperature, unlike Taq which has some activity at lower temperatures too
- Can degrade oligonucleotides, causing smearing (on gel).
