Pfu
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(Difference between revisions)
(New page: ==Purpose== *Pfu is a thermostable DNA polymerase that can be used for PCR. ==Procurement== *You can buy Pfu for example at [[http://www.promega.com/catalog/catalogproducts.aspx?categoryn...) |
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==Procurement== | ==Procurement== | ||
| - | *You can buy Pfu for example at | + | *You can buy Pfu for example at [http://www.promega.com/catalog/catalogproducts.aspx?categoryname=productleaf_1311&ckt=1 Promega]. |
*NEB does for some reason not offer Pfu. | *NEB does for some reason not offer Pfu. | ||
Revision as of 04:26, 14 April 2008
Purpose
- Pfu is a thermostable DNA polymerase that can be used for PCR.
Procurement
- You can buy Pfu for example at Promega.
- NEB does for some reason not offer Pfu.
Use
- Pfu has proof reading activity which makes it more accurate than for example Taq.
- Pfu does not exhibit 5' -> 3' exonuclease activity, which prevents degradation of the primers in mutagenesis methods based on ligation of primers or elongated primers.
