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Lab Meetings Lab Meeting Schedule: Spring 21 Lab Meeting Schedule: Fall 20 Lab Meeting Schedule: Summer 20 Lab Meeting Schedule: Spring 20 Older Lab Meetings How to format your group meeting presentation Peyton Lab master 4:3 Peyton Lab master 16:9 Data Polio et al., Lung biomechanics Fellowship Opportunities Here are fellowship possibilities for grad students and post-docs in the Peyton lab. Alumni or current members that have had these fellowships are listed for you to contact. Links to several opportunities, updated often NSF GRFP won by Max Nowak and Alyssa Schwartz UMass NIH CBI T32 won by Lauren Barney and Elizabeth Brooks UMass NIH BTP T32 won by Ning-Hsuan Tseng UMass NSF SMLS-NRT NDSEG NIH F31 NIH F32 won by Shelly Peyton Lab Duties Lab Duty List Protocols General Lab Exit Policy Data Storage Protocol Manuscript Writing Guide Writing Guide Checklist Two Column Template Mouse Experiment Guide Figures/Tables Checklist Updating OpenWetWare Shipping Biological Materials Using Material Transfer Agreements Lulzbot 3D Printer Using FreeCAD to Create 3D Printable Objects Guidelines for using FIJI (ImageJ) Accessing Peyton Lab Server Mammalian Cell Culture Feeding Cells Splitting Cells Freezing Cells Spheroid Culture Spheroid Recovery Cell Extraction from ECM by Sam Polio Macrophage Isolation from Mice Cell Staining Primary Ovarian Cancer Ascites Culture for Drug Screening Experiments Making Cell Subclones Chemistry APTES-DSC Coverslips, Will Herrick APTES-DSC Coverslips, Erinn Dandley APTES and DSC Coverslip Treatment (old, SPeyton) Carbonate Coating of TCPS Methacyrlate Coverslip Treatment PEG Acrylation Protein Adsorption on 96-well plates APTES-DSC Coverslips, Lauren Barney APTES-DSC Coverslips & 96-well plates Peptide Synthesis Solid phase peptide synthesis by Aritra Nath Kundu HPLC for peptide purification by Sualyneth Galarza MALDI for peptide characterization by Maria Gencoglu Hydrogels Crosby Polymer Microlenses, by D. Ryman 3D Swelling Assays Making PEG-Fibrinogen macromers 3D Non-degradable PEG-RGD gels Inverse-Opal PEG gels RGD reaction on Porous PEG gels Polyacrylamide Gels Polyacrylamide gels on coverslips for microscopy PEG-PC Gels on Coverslips or 96-well plates PEG-PC Gels on Coverslips or 96-well plates, updated 8/28/17 3D PEG-MAL Gels Protein/peptide functionalization of PEG-PC gels RNA extraction from PEG hydrogels Microscopy and Analysis Vivatome User's Guide Vivatome Log Sheets Tracking cells in Imaris by D. Ryman, L. Barney Making Kymographs in ImageJ Manual Tracking for Migration in ImageJ Boyden Chamber Invasion Assay Microscope Guidelines by Carey Dougan FIJI Overview by Carey Dougan and Aritra Nath Kundu Molecular Biology MAGPix User's Guide Western Blot Protocol by Alyssa Schwartz Phage Display Protocol by Marcos Manganare Whole Exome Sequencing Protocol by Alyssa Schwartz QPCR protocol by Sualyneth Galarza Mechanical Testing Mechanical Testing for Cavitation, Uniaxial Testing, & Rheology by Sam Polio Contact Adhesion Testing (CAT) for PEG-Maleimide hydrogels Microcontact Printing Subtractive Printing by Sam Polio Microcontact Printing Fibronectin by Sam Polio MATLAB Codes Proliferation Counter from Barney et al., 2017 Manuscript ‎ Traction Force Microscopy Codes link Heat map clustering Jansen et al., 2018 Manuscript ‎ Automated Liquid Handling Robotics Basic Biomek Protocol Important Dates AIChE October 28th-November 2nd, 2012, Pittsburgh, PA link Group Photos Tanglewood Summer 11