Peyton:Internal: Difference between revisions
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__NOTOC__ | __NOTOC__ | ||
==Lab Meetings== | ==Lab Meetings== | ||
*[[Peyton:Lab Meeting Schedule | *[[Peyton:Lab Meeting Schedule Spring 2021|'''Lab Meeting Schedule: Spring 21''']] | ||
*[[Peyton:Lab Meeting Schedule | *[[Peyton:Lab Meeting Schedule Fall 2020|'''Lab Meeting Schedule: Fall 20''']] | ||
*[[ | *[[Peyton:Lab Meeting Schedule Summer 2020|'''Lab Meeting Schedule: Summer 20''']] | ||
*[[Peyton:Lab Meeting Schedule Spring | *[[Peyton:Lab Meeting Schedule Spring 2020|'''Lab Meeting Schedule: Spring 20''']] | ||
*[[Peyton:Lab | *[[Peyton:Old Lab Meetings|'''Older Lab Meetings''']]<br> | ||
*[[ | *[[Media:Peyton_Meeting_Format.jpg |How to format your group meeting presentation]]<br> | ||
*[[ | *[[Media:Peyton_Lab_slide_Master.pptx.zip | Peyton Lab master 4:3]]<br> | ||
*[[Media: | *[[Media:Peyton_Lab_slide_Master.pptx.zip | Peyton Lab master 16:9]]<br> | ||
== | ==Data== | ||
*[[ | *[[Peyton:Polio et al., Lung biomechanics|'''Polio et al., Lung biomechanics''']]<br> | ||
== Fellowship Opportunities == | == Fellowship Opportunities == | ||
Here are fellowship possibilities for grad students and post-docs in the Peyton lab. Alumni or current members that have had these fellowships are listed for you to contact. <br> | Here are fellowship possibilities for grad students and post-docs in the Peyton lab. Alumni or current members that have had these fellowships are listed for you to contact. <br> | ||
[https://research.jhu.edu/funding-opportunities/graduate/ Links to several opportunities, updated often]<br> | |||
[http://www.nsfgrfp.org/ NSF GRFP] won by Max Nowak and Alyssa Schwartz<br> | [http://www.nsfgrfp.org/ NSF GRFP] won by Max Nowak and Alyssa Schwartz<br> | ||
[http://www.umass.edu/cbi/traineeships.html UMass NIH CBI T32] won by Lauren Barney<br> | [http://www.umass.edu/cbi/traineeships.html UMass NIH CBI T32] won by Lauren Barney and Elizabeth Brooks<br> | ||
[https://btp.umass.edu/traineeships/ UMass NIH BTP T32] won by Ning-Hsuan Tseng<br> | |||
[http://www.smls-nrt.org UMass NSF SMLS-NRT]<br> | |||
[http://ndseg.asee.org/ NDSEG]<br> | [http://ndseg.asee.org/ NDSEG]<br> | ||
[http://grants.nih.gov/grants/funding/ac_search_results.htm?text_curr=f31&Search_Type=Activity NIH F31]<br> | [http://grants.nih.gov/grants/funding/ac_search_results.htm?text_curr=f31&Search_Type=Activity NIH F31]<br> | ||
[http://grants.nih.gov/training/nrsa.htm NIH F32] won by Shelly Peyton<br> | [http://grants.nih.gov/training/nrsa.htm NIH F32] won by Shelly Peyton<br> | ||
==Lab Duties== | ==Lab Duties== | ||
*[[Media: | *[[Media:PeytonLabTasks2018F.pdf |Lab Duty List]]<br> | ||
==Protocols== | ==Protocols== | ||
'''General''' | |||
*[[Media:Peyton_Exit.doc |Lab Exit Policy]]<br> | |||
*[[Media:Peyton_DataSharing.pdf |Data Storage Protocol]]<br> | |||
*[[Media:Peyton_Writing.doc |Manuscript Writing Guide]]<br> | |||
*[[Media:WritingGuideChecklist.docx |Writing Guide Checklist]]<br> | |||
*[[Media:TwoColumnTemplate.docx |Two Column Template]]<br> | |||
*[[Media:Peyton_Mouse.docx |Mouse Experiment Guide]]<br> | |||
*[[Media:20170315_FigureChecklist.docx |Figures/Tables Checklist]]<br> | |||
*[[Media:Updating_OpenWetWare.pdf |Updating OpenWetWare]]<br> | |||
*[[Media:Biological Materials Shipping Protocol.pdf |Shipping Biological Materials]]<br> | |||
*[[Media:MTA Shipping Protocol.pdf |Using Material Transfer Agreements]]<br> | |||
*[[Media:3D_Printing_Protocol.pdf |Lulzbot 3D Printer]]<br> | |||
*[[Media:FreeCAD protocol for 3D Printing.pdf |Using FreeCAD to Create 3D Printable Objects]]<br> | |||
*[[Media:FIJI_(ImageJ)_Guidelines.pdf |Guidelines for using FIJI (ImageJ)]]<br> | |||
*[[Media:Access_the_Peyton_Lab_Server_Protocol.pdf |Accessing Peyton Lab Server]]<br> | |||
'''Mammalian Cell Culture''' | '''Mammalian Cell Culture''' | ||
*[[Media: | *[[Media:Peyton_Feeding_Adherent_Cells.doc |Feeding Cells]]<br> | ||
*[[Media:Peyton_Splitting.doc |Splitting Cells]]<br> | *[[Media:Peyton_Splitting.doc |Splitting Cells]]<br> | ||
*[[Media:Peyton_Freezing.doc |Freezing Cells]]<br> | *[[Media:Peyton_Freezing.doc |Freezing Cells]]<br> | ||
*[[Media:Spheroid-Culture_Protocol.docx |Spheroid Culture]]<br> | |||
*[[Media:Spheroid-Recovery_Protocol.docx |Spheroid Recovery]]<br> | |||
*[[Media:Extraction_Buffer.docx | Cell Extraction from ECM by Sam Polio]]<br> | |||
*[[Media:Macrophage_Isolation_Protocol.docx | Macrophage Isolation from Mice]]<br> | |||
*[[Media:Cell_Staining_(Ki67_&_live-dead).docx | Cell Staining]]<br> | |||
*[[Media:Primary_Ovarian_Cancer_Ascites_Culture_for_Drug_Screening_Experiments_EB.pdf | Primary Ovarian Cancer Ascites Culture for Drug Screening Experiments]]<br> | |||
*[[Media:Making cell subclones Protocol.pdf | Making Cell Subclones]]<br> | |||
'''Chemistry''' | '''Chemistry''' | ||
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*[[Media:Peyton_PEGDA.doc |PEG Acrylation]]<br> | *[[Media:Peyton_PEGDA.doc |PEG Acrylation]]<br> | ||
*[[Media:Peyton_Adsorption.doc |Protein Adsorption on 96-well plates]]<br> | *[[Media:Peyton_Adsorption.doc |Protein Adsorption on 96-well plates]]<br> | ||
*[[Media:APTES-DSC_protocol_7-11-16.docx |APTES-DSC Coverslips, Lauren Barney]]<br> | |||
*[[Media:APTES_DSC_protocol_011618.pdf |APTES-DSC Coverslips & 96-well plates]]<br> | |||
'''Peptide Synthesis''' | |||
*[[Media:Peptide Synthesis_ANK_20190506.pdf | Solid phase peptide synthesis by Aritra Nath Kundu]]<br> | |||
*[[Media:HPLC_protocol_for_peptide_purification_by_Sualyneth_Galarza.pdf | HPLC for peptide purification by Sualyneth Galarza]]<br> | |||
*[[Media:MALDI_protocol.pdf |MALDI for peptide characterization by Maria Gencoglu]]<br> | |||
'''Hydrogels''' | '''Hydrogels''' | ||
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*[[Media:Peyton_PAA.doc |Polyacrylamide Gels]]<br> | *[[Media:Peyton_PAA.doc |Polyacrylamide Gels]]<br> | ||
*[[Media:PAA_gels_on_coverslips.docx |Polyacrylamide gels on coverslips for microscopy]]<br> | *[[Media:PAA_gels_on_coverslips.docx |Polyacrylamide gels on coverslips for microscopy]]<br> | ||
*[[Media:PEG- | *[[Media:PEG-PC_Gel_Protocol.docx |PEG-PC Gels on Coverslips or 96-well plates]]<br> | ||
*[[Media:PEG_PC_Gel_Protocol.pdf |PEG-PC Gels on Coverslips or 96-well plates, updated 8/28/17]]<br> | |||
*[[Media:PEG-MAL_Hydrogel_protocol_(1).docx |3D PEG-MAL Gels]]<br> | |||
*[[Media:Proteins_peptide_functionalization_of_PEG-PC_gels_on_coverslips_or_96-well_plates.pdf |Protein/peptide functionalization of PEG-PC gels]]<br> | |||
*[[Media:RNA extraction from gels_HK_20170918_v8.pdf |RNA extraction from PEG hydrogels]]<br> | |||
'''Microscopy''' | '''Microscopy and Analysis''' | ||
*[[Media:Zeiss_Vivatome_Users_Guide.docx |Vivatome User's Guide]]<br> | *[[Media:Zeiss_Vivatome_Users_Guide.docx |Vivatome User's Guide]]<br> | ||
*[[Media:Vivatome_Log_book.xlsx |Vivatome Log Sheets]]<br> | *[[Media:Vivatome_Log_book.xlsx |Vivatome Log Sheets]]<br> | ||
*[[Media: | *[[Media:IMARIS_tracking_LEB.docx |Tracking cells in Imaris by D. Ryman, L. Barney]]<br> | ||
*[[Media:ImageJ_Kymograph_instructions.pptx |Making Kymographs in ImageJ]]<br> | |||
*[[Media:Manual_Tracking_in_ImageJ.pptx |Manual Tracking for Migration in ImageJ]]<br> | |||
*[[Media:Invasion Assay.docx |Boyden Chamber Invasion Assay]]<br> | |||
*[[Media:Microscopy Guidelines_2.pdf |Microscope Guidelines by Carey Dougan]]<br> | |||
*[[Media:FIJIReviewGroupMeeting.pdf | FIJI Overview by Carey Dougan and Aritra Nath Kundu]]<br> | |||
''' | '''Molecular Biology''' | ||
*[[Media:MAGPix_Assay_Notes.docx |MAGPix User's Guide]]<br> | *[[Media:MAGPix_Assay_Notes.docx |MAGPix User's Guide]]<br> | ||
*[[Media:Western Blot protocol.docx |Western Blot Protocol by Alyssa Schwartz]]<br> | |||
*[[Media:Manganare Phage Display.pdf |Phage Display Protocol by Marcos Manganare]]<br> | |||
*[[Media:WXS_Protocol.docx|Whole Exome Sequencing Protocol by Alyssa Schwartz]]<br> | |||
*[[Media:QPCR_Protocol_by_Sualyneth_Galarza.pdf|QPCR protocol by Sualyneth Galarza]]<br> | |||
'''Mechanical Testing''' | |||
*[[Media:Lung_Mechanical_Testing_Procedures.docx |Mechanical Testing for Cavitation, Uniaxial Testing, & Rheology by Sam Polio]]<br> | |||
*[[Media:PEG-Maleimide mechanical testing (CAT)_HK_20180507_v3.pdf |Contact Adhesion Testing (CAT) for PEG-Maleimide hydrogels]]<br> | |||
''' | '''Microcontact Printing''' | ||
*[[Media: | *[[Media:Procedure_for_Subtractive_Stamping.docx | Subtractive Printing by Sam Polio]]<br> | ||
*[[Media: | *[[Media:Protocol_for_Microcontact_Printing_Fibronectin.docx | Microcontact Printing Fibronectin by Sam Polio]]<br> | ||
'''MATLAB Codes''' | |||
*[[Media:ProPro.m | Proliferation Counter from Barney et al., 2017 Manuscript ]] | |||
*Traction Force Microscopy Codes [https://github.com/PeytonLab/3DTFM link]<br> | |||
*[[Media:FBR_heatmap_and_dendrogram.pdf | Heat map clustering Jansen et al., 2018 Manuscript ]] | |||
'''Automated Liquid Handling Robotics''' | |||
*[[Media:Basic Biomek Protocol.pdf | Basic Biomek Protocol]]<br> | |||
== Important Dates == | == Important Dates == | ||
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*[[Peyton:Tanglewood Summer 11|'''Tanglewood Summer 11''']]<br> | *[[Peyton:Tanglewood Summer 11|'''Tanglewood Summer 11''']]<br> | ||
[[File:LabChicken.jpg|200px|Description of image]] | |||
</div> | </div> | ||
|} | |} |