Pecinka lab:Protocols

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 12: Line 12:
[http://www.plantcell.org/cgi/reprint/20/7/1736 <font style="color:#000;">Eleven Golden Rules of qRT-PCR (Uvardi et al.)</font>]
[http://www.plantcell.org/cgi/reprint/20/7/1736 <font style="color:#000;">Eleven Golden Rules of qRT-PCR (Uvardi et al.)</font>]
-
RNA work
+
'''RNA work'''
-
[[media:RNA_gels.pdf| RNA gels]]
+
Trizol extraction [[media:TRIZOL.pdf| (PDF)]]
-
[[Pecinka_Lab:Trizol|<font style="color:#000;">Trizol extraction</font>]]
+
RNA gels [[media:RNA_gels.pdf| (PDF)]]
|width=350px style="padding: 5px; background-color: #ffffff; border: 0px solid #C9D3EB;" |
|width=350px style="padding: 5px; background-color: #ffffff; border: 0px solid #C9D3EB;" |

Revision as of 12:24, 18 February 2013

Image:LablogoAP2.tif


Home      Research      People      Publications      Protocols      Resources      Seminars      Positions      Lab life     


Protocols

Miniprep using Promega reagents but not the columns

MS plates for plant growth

Eleven Golden Rules of qRT-PCR (Uvardi et al.)

RNA work

Trizol extraction (PDF)

RNA gels (PDF)

Stocks

Antibiotics

Electrophoresis Buffers

Loading Dye and 1kb Ladder

PCR taq, buffer and MgCl2

Lab Safety

Pecinka lab safety

Lab Tasks

Scripts, instructions and miscellaneous

Analyzing luminiscence over time with ImageJ

HALLE 14 growth chambers


Personal tools