Pecinka lab:Protocols

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Max Planck Institute for Plant Breeding Research
Department of Plant Breeding and Genetics
Carl-von-Linné-Weg 10
Cologne, DE-508 29
GERMANY

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Protocols

Miniprep using Promega reagents but not the columns

MS plates for plant growth

Eleven Golden Rules of qRT-PCR (Uvardi et al.)

RNA work

RNeasy kit extraction (PDF)

Trizol extraction (PDF)

RNA gels (PDF)

Stocks

Electrophoresis Buffers

Loading Dye and 1kb Ladder

PCR taq, buffer and MgCl₂

Lab Safety

Pecinka lab safety

Lab Tasks

Scripts, instructions and miscellaneous

Analyzing luminiscence over time with ImageJ

HALLE 14 growth chambers

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Ales Pecinka