Pecinka lab:Protocols

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Current revision (11:19, 17 July 2013) (view source)
 
(One intermediate revision not shown.)
Line 13: Line 13:
'''RNA work'''
'''RNA work'''
 +
 +
RNeasy kit extraction [[media:RNeasy_plant.pdf| (PDF)]]
Trizol extraction [[media:TRIZOL.pdf| (PDF)]]
Trizol extraction [[media:TRIZOL.pdf| (PDF)]]
RNA gels [[media:RNA_gels.pdf| (PDF)]]
RNA gels [[media:RNA_gels.pdf| (PDF)]]
 +
 +
'''Histochemical staining'''
 +
 +
GUS staining [[media:GUS_staining_OWW.pdf| (PDF)]]
 +
 +
Alexander staining for pollen viability [[media:Alexander_staining.pdf| (PDF)]]
|width=350px style="padding: 5px; background-color: #ffffff; border: 0px solid #C9D3EB;" |
|width=350px style="padding: 5px; background-color: #ffffff; border: 0px solid #C9D3EB;" |

Current revision

Image:LablogoAP2.tif


Home      Research      People      Publications      Protocols      Resources      Seminars      Positions      Lab life     


Protocols

Miniprep using Promega reagents but not the columns

MS plates for plant growth

Eleven Golden Rules of qRT-PCR (Uvardi et al.)

RNA work

RNeasy kit extraction (PDF)

Trizol extraction (PDF)

RNA gels (PDF)

Histochemical staining

GUS staining (PDF)

Alexander staining for pollen viability (PDF)

Stocks

Antibiotics

Electrophoresis Buffers

Loading Dye and 1kb Ladder

PCR taq, buffer and MgCl2

Lab Safety

Pecinka lab safety

Lab Tasks

Scripts, instructions and miscellaneous

Analyzing luminiscence over time with ImageJ

HALLE 14 growth chambers


Personal tools