PMSF - Revision history

Tk: /* Purpose */

Tk — Sat, 08 Jan 2011 23:04:36 GMT

Purpose

←Older revision		Revision as of 23:04, 8 January 2011
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	==Purpose==		==Purpose==
-	PMSF stands for Phenylmethylsulfonyl fluoride. It is used as an irreversible inhibitor of serine protease activity for example in protein purification or co-immunoprecipitation.	+	PMSF stands for Phenylmethylsulfonyl fluoride. It is used as an irreversible inhibitor of serine protease activity for example in protein purification or co-immunoprecipitation. It has no effect on other proteases that might be present. It can also inactivate some non-proteolytic enzymes that contain serine in the active site.

	Molecular W.: 174.19		Molecular W.: 174.19

Tk: /* Safety */

Tk — Sat, 08 Jan 2011 23:02:44 GMT

Safety

←Older revision		Revision as of 23:02, 8 January 2011
Line 13:		Line 13:

	==Safety==		==Safety==
-	PMSF is poisonous and should be handled carefully.	+	PMSF is extremely poisonous and should be handled carefully.


	[[Category:Material]] [[Category:Chemical]]		[[Category:Material]] [[Category:Chemical]]

Tk: /* Use */

Tk — Sat, 08 Jan 2011 23:02:04 GMT

Use

←Older revision		Revision as of 23:02, 8 January 2011
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	==Use==		==Use==
-	PMSF is not soluble in water, dissolve in ethanol or methanol.	+	PMSF is not soluble in water, dissolve in isopropanol, ethanol or methanol.
-	200 mM stock solution (0.035 g/mL) is stable for months at 4°C.	+	200 mM stock solution (0.035 g/mL) is stable for months at 4°C, although storage at -20 is probably better.
-	A common final concentration of PMSF is 1mM of ~~course depending on the experimental setup~~.	+	A common final concentration of PMSF is 1mM. PMSF is extremely toxic, and preparation of solutions should be done very carefully. PMSF is not stable in alkaline conditions, or in water. For disposal, add alkali in water and wait to dispose.

	==Safety==		==Safety==

Tk: /* Purpose */

Tk — Sat, 08 Jan 2011 22:59:15 GMT

Purpose

←Older revision		Revision as of 22:59, 8 January 2011
Line 1:		Line 1:
	==Purpose==		==Purpose==
-	PMSF stands for Phenylmethylsulfonyl fluoride. It is used as an inhibitor of protease activity for example in protein purification or co-immunoprecipitation.	+	PMSF stands for Phenylmethylsulfonyl fluoride. It is used as an irreversible inhibitor of serine protease activity for example in protein purification or co-immunoprecipitation.

	Molecular W.: 174.19		Molecular W.: 174.19

Eric Milliman: fixed typos

Eric Milliman — Tue, 01 Sep 2009 22:27:54 GMT

fixed typos

←Older revision		Revision as of 22:27, 1 September 2009
Line 1:		Line 1:
	==Purpose==		==Purpose==
-	PMSF stands for Phenylmethylsulfonyl fluoride. It is used as an inhibitor of protease activity for example in protein ~~purifications~~ or ~~coimunoprecipitations~~.	+	PMSF stands for Phenylmethylsulfonyl fluoride. It is used as an inhibitor of protease activity for example in protein purification or co-immunoprecipitation.

	Molecular W.: 174.19		Molecular W.: 174.19
Line 8:		Line 8:

	==Use==		==Use==
-	~~Don't try solution~~ in water, ~~it does not work. Take~~ ethanol or methanol.	+	PMSF is not soluble in water, dissolve in ethanol or methanol.
-	200 mM stock solution ~~are~~ stable for months at 4°C.	+	200 mM stock solution (0.035 g/mL) is stable for months at 4°C.
-	A ~~normal endconcentration~~ of PMSF is ~~probably about~~ 1mM of course depending on the experimental setup.	+	A common final concentration of PMSF is 1mM of course depending on the experimental setup.

	==Safety==		==Safety==
-	PMSF is ~~poisenes~~ and should be ~~handeld~~ carefully.	+	PMSF is poisonous and should be handled carefully.


	[[Category:Material]] [[Category:Chemical]]		[[Category:Material]] [[Category:Chemical]]

Torsten Waldminghaus: added tag

Torsten Waldminghaus — Tue, 20 Nov 2007 11:00:11 GMT

added tag

←Older revision		Revision as of 11:00, 20 November 2007
Line 14:		Line 14:
	==Safety==		==Safety==
	PMSF is poisenes and should be handeld carefully.		PMSF is poisenes and should be handeld carefully.
		+
		+
		+	[[Category:Material]] [[Category:Chemical]]

Torsten Waldminghaus: New page: ==Purpose== PMSF stands for Phenylmethylsulfonyl fluoride. It is used as an inhibitor of protease activity for example in protein purifications or coimunoprecipitations. Molecular W.: 174...

Torsten Waldminghaus — Tue, 20 Nov 2007 08:41:45 GMT

New page: ==Purpose== PMSF stands for Phenylmethylsulfonyl fluoride. It is used as an inhibitor of protease activity for example in protein purifications or coimunoprecipitations. Molecular W.: 174...

New page

==Purpose==
PMSF stands for Phenylmethylsulfonyl fluoride. It is used as an inhibitor of protease activity for example in protein purifications or coimunoprecipitations.

Molecular W.: 174.19

==Procurement==
You can buy PMSF for example at [http://www.piercenet.com/ Pierce] or [http://www.sigmaaldrich.com Sigma].

==Use==
Don't try solution in water, it does not work. Take ethanol or methanol.
200 mM stock solution are stable for months at 4°C.
A normal endconcentration of PMSF is probably about 1mM of course depending on the experimental setup.

==Safety==
PMSF is poisenes and should be handeld carefully.