http://openwetware.org/index.php?title=PMSF&feed=atom&action=history 2013-05-22T15:10:48Z Revision history for this page on the wiki MediaWiki 1.13.2 http://openwetware.org/index.php?title=PMSF&diff=484262&oldid=prev 2011-01-08T23:04:36Z

<p><span class="autocomment">Purpose</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 23:04, 8 January 2011</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Purpose==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Purpose==</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>PMSF stands for Phenylmethylsulfonyl fluoride. It is used as an irreversible inhibitor of serine protease activity for example in protein purification or co-immunoprecipitation.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>PMSF stands for Phenylmethylsulfonyl fluoride. It is used as an irreversible inhibitor of serine protease activity for example in protein purification or co-immunoprecipitation<ins class="diffchange diffchange-inline">. It has no effect on other proteases that might be present.&nbsp; It can also inactivate some non-proteolytic enzymes that contain serine in the active site</ins>.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Molecular W.: 174.19</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Molecular W.: 174.19</div></td></tr> <!-- diff generator: internal 2013-05-22 06:07:01 --> <!-- diff cache key owwdb:diff:version:1.11a:oldid:484261:newid:484262 --> </table>

Tk http://openwetware.org/index.php?title=PMSF&diff=484261&oldid=prev 2011-01-08T23:02:44Z

<p><span class="autocomment">Safety</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 23:02, 8 January 2011</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 13:</td> <td colspan="2" class="diff-lineno">Line 13:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Safety==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Safety==</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>PMSF is poisonous and should be handled carefully.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>PMSF is <ins class="diffchange diffchange-inline">extremely </ins>poisonous and should be handled carefully.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category:Material]] [[Category:Chemical]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category:Material]] [[Category:Chemical]]</div></td></tr> <!-- diff generator: internal 2013-05-22 15:10:49 --> </table>

Tk http://openwetware.org/index.php?title=PMSF&diff=484260&oldid=prev 2011-01-08T23:02:04Z

<p><span class="autocomment">Use</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 23:02, 8 January 2011</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 8:</td> <td colspan="2" class="diff-lineno">Line 8:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Use==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Use==</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>PMSF is not soluble in water, dissolve in ethanol or methanol.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>PMSF is not soluble in water, dissolve in <ins class="diffchange diffchange-inline">isopropanol, </ins>ethanol or methanol.</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>200 mM stock solution (0.035 g/mL) is stable for months at 4°C.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>200 mM stock solution (0.035 g/mL) is stable for months at 4°C<ins class="diffchange diffchange-inline">, although storage at -20 is probably better</ins>.</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>A common final concentration of PMSF is 1mM of <del class="diffchange diffchange-inline">course depending on the experimental setup</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>A common final concentration of PMSF is 1mM<ins class="diffchange diffchange-inline">.&nbsp; PMSF is extremely toxic, and preparation </ins>of <ins class="diffchange diffchange-inline">solutions should be done very carefully.&nbsp; PMSF is not stable in alkaline conditions, or in water.&nbsp; For disposal, add alkali in water and wait to dispose</ins>.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Safety==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Safety==</div></td></tr> <!-- diff generator: internal 2013-05-22 15:10:49 --> </table>

Tk http://openwetware.org/index.php?title=PMSF&diff=484259&oldid=prev 2011-01-08T22:59:15Z

<p><span class="autocomment">Purpose</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 22:59, 8 January 2011</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Purpose==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Purpose==</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>PMSF stands for Phenylmethylsulfonyl fluoride. It is used as an inhibitor of protease activity for example in protein purification or co-immunoprecipitation.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>PMSF stands for Phenylmethylsulfonyl fluoride. It is used as an <ins class="diffchange diffchange-inline">irreversible </ins>inhibitor of <ins class="diffchange diffchange-inline">serine </ins>protease activity for example in protein purification or co-immunoprecipitation.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Molecular W.: 174.19</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Molecular W.: 174.19</div></td></tr> <!-- diff generator: internal 2013-05-22 15:10:49 --> </table>

Tk http://openwetware.org/index.php?title=PMSF&diff=346339&oldid=prev 2009-09-01T22:27:54Z

<p>fixed typos</p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 22:27, 1 September 2009</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Purpose==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Purpose==</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>PMSF stands for Phenylmethylsulfonyl fluoride. It is used as an inhibitor of protease activity for example in protein <del class="diffchange diffchange-inline">purifications </del>or <del class="diffchange diffchange-inline">coimunoprecipitations</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>PMSF stands for Phenylmethylsulfonyl fluoride. It is used as an inhibitor of protease activity for example in protein <ins class="diffchange diffchange-inline">purification </ins>or <ins class="diffchange diffchange-inline">co-immunoprecipitation</ins>.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Molecular W.: 174.19</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Molecular W.: 174.19</div></td></tr> <tr><td colspan="2" class="diff-lineno">Line 8:</td> <td colspan="2" class="diff-lineno">Line 8:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Use==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Use==</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Don't try solution </del>in water, <del class="diffchange diffchange-inline">it does not work. Take </del>ethanol or methanol.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">PMSF is not soluble </ins>in water, <ins class="diffchange diffchange-inline">dissolve in </ins>ethanol or methanol.</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>200 mM stock solution <del class="diffchange diffchange-inline">are </del>stable for months at 4°C.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>200 mM stock solution <ins class="diffchange diffchange-inline">(0.035 g/mL) is </ins>stable for months at 4°C.</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>A <del class="diffchange diffchange-inline">normal endconcentration </del>of PMSF is <del class="diffchange diffchange-inline">probably about </del>1mM of course depending on the experimental setup.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>A <ins class="diffchange diffchange-inline">common final concentration </ins>of PMSF is 1mM of course depending on the experimental setup.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Safety==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Safety==</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>PMSF is <del class="diffchange diffchange-inline">poisenes </del>and should be <del class="diffchange diffchange-inline">handeld </del>carefully.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>PMSF is <ins class="diffchange diffchange-inline">poisonous </ins>and should be <ins class="diffchange diffchange-inline">handled </ins>carefully.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category:Material]] [[Category:Chemical]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category:Material]] [[Category:Chemical]]</div></td></tr> <!-- diff generator: internal 2013-05-22 15:10:49 --> </table>

Eric Milliman http://openwetware.org/index.php?title=PMSF&diff=169111&oldid=prev 2007-11-20T11:00:11Z

<p>added tag</p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 11:00, 20 November 2007</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 14:</td> <td colspan="2" class="diff-lineno">Line 14:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Safety==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Safety==</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>PMSF is poisenes and should be handeld carefully.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>PMSF is poisenes and should be handeld carefully.</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Category:Material]] [[Category:Chemical]]</ins></div></td></tr> <!-- diff generator: internal 2013-05-22 15:10:49 --> </table>

Torsten Waldminghaus http://openwetware.org/index.php?title=PMSF&diff=169102&oldid=prev 2007-11-20T08:41:45Z

<p>New page: ==Purpose== PMSF stands for Phenylmethylsulfonyl fluoride. It is used as an inhibitor of protease activity for example in protein purifications or coimunoprecipitations. Molecular W.: 174...</p> <p><b>New page</b></p><div>==Purpose==<br /> PMSF stands for Phenylmethylsulfonyl fluoride. It is used as an inhibitor of protease activity for example in protein purifications or coimunoprecipitations.<br /> <br /> Molecular W.: 174.19<br /> <br /> ==Procurement==<br /> You can buy PMSF for example at [http://www.piercenet.com/ Pierce] or [http://www.sigmaaldrich.com Sigma].<br /> <br /> ==Use==<br /> Don't try solution in water, it does not work. Take ethanol or methanol.<br /> 200 mM stock solution are stable for months at 4°C.<br /> A normal endconcentration of PMSF is probably about 1mM of course depending on the experimental setup.<br /> <br /> ==Safety==<br /> PMSF is poisenes and should be handeld carefully.</div>

Torsten Waldminghaus