PMSF

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(Use)
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==Use==
==Use==
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PMSF is not soluble in water, dissolve in ethanol or methanol.
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PMSF is not soluble in water, dissolve in isopropanol, ethanol or methanol.
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200 mM stock solution (0.035 g/mL) is stable for months at 4°C.
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200 mM stock solution (0.035 g/mL) is stable for months at 4°C, although storage at -20 is probably better.
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A common final concentration of PMSF is 1mM of course depending on the experimental setup.
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A common final concentration of PMSF is 1mM.  PMSF is extremely toxic, and preparation of solutions should be done very carefully.  PMSF is not stable in alkaline conditions, or in water.  For disposal, add alkali in water and wait to dispose.
==Safety==
==Safety==

Revision as of 18:02, 8 January 2011

Contents

Purpose

PMSF stands for Phenylmethylsulfonyl fluoride. It is used as an irreversible inhibitor of serine protease activity for example in protein purification or co-immunoprecipitation.

Molecular W.: 174.19

Procurement

You can buy PMSF for example at Pierce or Sigma.

Use

PMSF is not soluble in water, dissolve in isopropanol, ethanol or methanol. 200 mM stock solution (0.035 g/mL) is stable for months at 4°C, although storage at -20 is probably better. A common final concentration of PMSF is 1mM. PMSF is extremely toxic, and preparation of solutions should be done very carefully. PMSF is not stable in alkaline conditions, or in water. For disposal, add alkali in water and wait to dispose.

Safety

PMSF is poisonous and should be handled carefully.

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