PLS

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(Making 2x PLS)
(Making 2x PLS)
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When ready to use, thaw and add Beta metcaptoethanol to 10% (volume/volume)
When ready to use, thaw and add Beta metcaptoethanol to 10% (volume/volume)
Then dilute to 1x with H20
Then dilute to 1x with H20
-
Now ready to use.
 

Revision as of 11:58, 31 August 2006

Making 2x PLS

  • 1M Tris pH 6.8 1.5ml
  • 10% SDS 6.0ml
  • Glycerol 3.0ml
  • H20 3.0ml
  • Bromophenol blue small dash to color

vortex, aliquot and freeze at -20

When ready to use, thaw and add Beta metcaptoethanol to 10% (volume/volume) Then dilute to 1x with H20

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