PLS: Difference between revisions
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== Making 2x PLS == | == Making 2x PLS == | ||
1M Tris pH 6.8 1.5ml | 1M Tris pH 6.8 1.5ml | ||
10% SDS 6.0ml | 10% SDS 6.0ml | ||
Glycerol 3.0ml | Glycerol 3.0ml | ||
H20 3.0ml | H20 3.0ml | ||
Bromophenol blue small dash to color | Bromophenol blue small dash to color | ||
Revision as of 08:57, 31 August 2006
Making 2x PLS
1M Tris pH 6.8 1.5ml
10% SDS 6.0ml
Glycerol 3.0ml
H20 3.0ml
Bromophenol blue small dash to color
vortex, aliquot and freeze at -20
When ready to use, thaw and add Beta metcaptoethanol to 10% (volume/volume) Then dilute to 1x with H20 Now ready to use.
