Revision as of 10:57, 31 August 2006
Making 2x PLS
1M Tris pH 6.8 1.5ml 10% SDS 6.0ml Glycerol 3.0ml H20 3.0ml Bromophenol blue small dash to color
vortex, aliquot and freeze at -20
When ready to use, thaw and add Beta metcaptoethanol to 10% (volume/volume) Then dilute to 1x with H20 Now ready to use.