PLS

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(Making 2x PLS)
(Making 2x PLS)
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1M Tris pH 6.8          1.5ml
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*1M Tris pH 6.8          1.5ml
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*10% SDS                6.0ml
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10% SDS                6.0ml
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*Glycerol                3.0ml
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*H20                    3.0ml
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Glycerol                3.0ml
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*Bromophenol blue        small dash to color
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+
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H20                    3.0ml
+
-
 
+
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Bromophenol blue        small dash to color
+
vortex, aliquot and freeze at -20
vortex, aliquot and freeze at -20

Revision as of 10:58, 31 August 2006

Making 2x PLS

  • 1M Tris pH 6.8 1.5ml
  • 10% SDS 6.0ml
  • Glycerol 3.0ml
  • H20 3.0ml
  • Bromophenol blue small dash to color

vortex, aliquot and freeze at -20

When ready to use, thaw and add Beta metcaptoethanol to 10% (volume/volume) Then dilute to 1x with H20 Now ready to use.

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