PLS

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(Making PLS)
Current revision (16:22, 11 March 2008) (view source)
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== Making 2x PLS ==
== Making 2x PLS ==
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1M Tris pH 6.8          1.5ml
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*1M Tris pH 6.8          1.5ml
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10% SDS                6.0ml
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*10% SDS                6.0ml
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Glycerol                3.0ml
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*Glycerol                3.0ml
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H20                    3.0ml
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*H20                    3.0ml
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Bromophenol blue        small dash to color
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*Bromophenol blue        small dash to color
vortex, aliquot and freeze at -20
vortex, aliquot and freeze at -20
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When ready to use, thaw and add Beta metcaptoethanol to 10% (volume/volume)
When ready to use, thaw and add Beta metcaptoethanol to 10% (volume/volume)
Then dilute to 1x with H20
Then dilute to 1x with H20
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Now ready to use.
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[[Category:Material]] and [[Category:PAGE]]

Current revision

Making 2x PLS

  • 1M Tris pH 6.8 1.5ml
  • 10% SDS 6.0ml
  • Glycerol 3.0ml
  • H20 3.0ml
  • Bromophenol blue small dash to color

vortex, aliquot and freeze at -20

When ready to use, thaw and add Beta metcaptoethanol to 10% (volume/volume) Then dilute to 1x with H20 and

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