PIPES

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* usable buffering range of 6.1 to 7.5
* usable buffering range of 6.1 to 7.5
* molecular weight 302.4
* molecular weight 302.4
-
* &Delta;pK<sub>a</sub>/&Delta;T = .0085
+
* &Delta;pK<sub>a</sub>/&Delta;T = -.0085
Buffers are typically 0.5 M, prepared by neutralizing PIPES with sodium hydroxide.  PIPES is essentially insoluble until it is neutralized.  
Buffers are typically 0.5 M, prepared by neutralizing PIPES with sodium hydroxide.  PIPES is essentially insoluble until it is neutralized.  

Revision as of 18:07, 14 April 2006

piperazine-N,Nā€™-bis(ethanesulfonic acid); 1,4-piperazinediethanesulfonic acid, CAS NUMBER: 5625-37-6, Sigma P6757

A Good buffer [1][2].

  • pKa at 25C of 6.8 (a second one at pH 3 is not of interest)
  • usable buffering range of 6.1 to 7.5
  • molecular weight 302.4
  • ΔpKa/ΔT = -.0085

Buffers are typically 0.5 M, prepared by neutralizing PIPES with sodium hydroxide. PIPES is essentially insoluble until it is neutralized.

  1. Good NE, Winget GD, Winter W, Connolly TN, Izawa S, and Singh RM. . pmid:5942950. PubMed HubMed [Good66]
  2. Blanchard JS. . pmid:6717292. PubMed HubMed [Blanchard84]
All Medline abstracts: PubMed HubMed
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