PCR inhibitors

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== See also ==
== See also ==
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* [[Polymerase chain reaction inhibitors|PCR inhibitor at the Wikipedia]]
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* [http://en.wikipedia.org/wiki/Polymerase_chain_reaction_inhibitors PCR inhibitor at the Wikipedia]
* [[Maheshri:PCR]] - has some tips on how to avoid inhibition
* [[Maheshri:PCR]] - has some tips on how to avoid inhibition

Revision as of 07:21, 7 July 2010

Schematic of competitive inhibition of an enzyme. Note that active site inhibition is only one of many reasons why a PCR may not work.
Schematic of competitive inhibition of an enzyme. Note that active site inhibition is only one of many reasons why a PCR may not work.

Many compounds also contained in the PCR template beside the target DNA can interfere with the polymerisation of nucleotides by the Taq or a related polymerase. This may result in false negative results, i.e. no PCR product even though the template is present.

Contents

Inhibitors from the purification process

Inhibitors from the source tissue

  • components of blood: heme, hemoglobin, lactoferrin, immunoglobin G (IgG)
  • liver, digestive tract, feces: bile salts, polysaccharides
  • connective tissue, skin: collagen, melanin
  • urine: urea

See Rådström et al. 2004 (PMID 14764939) for a review on how to avoid inhibition from tissue components.

Template inhibition

Since inhibitors often enter the PCR reaction via the template preparation, decreasing template volume may improve PCR results indirectly by reducing the quantity of inhibitors.

See also

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