PCR inhibitors: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Line 11: | Line 11: | ||
== Inhibitors from the source tissue == | == Inhibitors from the source tissue == | ||
* components of blood: heme, hemoglobin, lactoferrin, immunoglobin G (IgG) | |||
* liver, digestive tract, feces: bile salts, polysaccharides | |||
* connective tissue, skin: collagen, melanin | |||
* urine: urea | |||
See Rådström et al. 2004 (PMID 14764939) for a review on how to avoid inhibition from tissue components. | |||
== Template inhibition == | == Template inhibition == |
Revision as of 04:30, 7 July 2010
Many compounds also contained in the PCR template beside the target DNA can interfere with the polymerisation of nucleotides by the Taq or a related polymerase. This may result in false negative results, i.e. no PCR product even though the template is present.
Inhibitors from the purification process
- alcohols like ethanol and 2-propanol (isopropanol) from DNA template precipitation (Qiagen test of sequencing inhibition by Welters et al. 1997)
- organic solvents like phenol from phenol/chloroform purification (PMID 8136148)
- salts like KCl, NaCl from precipitation
- detergents like SDS,.. from membrane lysis (PMID 2223070)
Inhibitors from the source tissue
- components of blood: heme, hemoglobin, lactoferrin, immunoglobin G (IgG)
- liver, digestive tract, feces: bile salts, polysaccharides
- connective tissue, skin: collagen, melanin
- urine: urea
See Rådström et al. 2004 (PMID 14764939) for a review on how to avoid inhibition from tissue components.