PBST - Revision history

Etienne Robillard: /* Purpose */

Etienne Robillard — Mon, 24 Dec 2012 01:42:00 GMT

Purpose

←Older revision		Revision as of 01:42, 24 December 2012
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	== Purpose ==		== Purpose ==
-	* PBST (Triton) is for example used for washing in immunohistology. A 1% PBS Triton X-100 wash can also be added to fixed tissue sections for 10 minutes (followed by 3 ~~time,~~ 15~~-minute~~ PBS wash) to permeabilize tissue prior to blocking at the beginning of a immunostaining experiment.	+	* PBST (Triton) is for example used for washing in immunohistology. A 1% PBS Triton X-100 wash can also be added to fixed tissue sections for 10 minutes followed by 3 x 15 minutes PBS wash to permeabilize tissue prior to blocking at the beginning of a immunostaining experiment.
-		+
	* PBST is used in [[protein blots]] for washing of membrane labelled with antibody.		* PBST is used in [[protein blots]] for washing of membrane labelled with antibody.

Jakob Suckale: merged with content from PBS-T page, now an empty redirect

Jakob Suckale — Tue, 05 May 2009 13:15:56 GMT

merged with content from PBS-T page, now an empty redirect

←Older revision		Revision as of 13:15, 5 May 2009
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-	~~==Purpose==~~	+	[[PBS]] stands for phosphate buffered saline. Adding of the detergent Tween 20 or Triton X-100 makes it PBST (people use the name PBST/PBS-T for both).
-	* [[PBS]] stands for phosphate buffered saline. Adding of the detergent Tween 20 or Triton X-100 makes it PBST (people use the name PBST ~~for both).~~	+
-	*PBST (Triton) is for example used for washing in immunohistology. A 1% PBS ~~Triton X~~-~~100 wash can also be added to fixed tissue sections~~ for ~~10 minutes (followed by 3 time, 15-minute PBS wash~~) ~~to permeabilize tissue prior to blocking at the beginning of a immunostaining experiment~~.	+

-	==~~Recipe~~==	+	== Purpose ==
-	*~~See~~ [[PBS]]	+	* PBST (Triton) is for example used for washing in immunohistology. A 1% PBS Triton X-100 wash can also be added to fixed tissue sections for 10 minutes (followed by 3 time, 15-minute PBS wash) to permeabilize tissue prior to blocking at the beginning of a immunostaining experiment.
		+
		+	* PBST is used in [[protein blots]] for washing of membrane labelled with antibody.
		+
		+	== Recipe ==
		+	0.1% (v/v) Tween 20 in 1x [[PBS]]
		+
		+	examples
		+	* 100µl in 100ml 1x PBS
		+	* 1ml in 1L 1xPBS

	[[Category:Material]] [[Category:Buffers]]		[[Category:Material]] [[Category:Buffers]]

Torsten Waldminghaus at 14:41, 12 January 2009

Torsten Waldminghaus — Mon, 12 Jan 2009 14:41:57 GMT

←Older revision		Revision as of 14:41, 12 January 2009
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	==Purpose==		==Purpose==
-	* [[PBS]] stands for phosphate buffered saline. Adding of the detergent Tween 20 or ~~TritonX100~~ makes it PBST (people use the name PBST for both).	+	* [[PBS]] stands for phosphate buffered saline. Adding of the detergent Tween 20 or Triton X-100 makes it PBST (people use the name PBST for both).
	*PBST (Triton) is for example used for washing in immunohistology. A 1% PBS Triton X-100 wash can also be added to fixed tissue sections for 10 minutes (followed by 3 time, 15-minute PBS wash) to permeabilize tissue prior to blocking at the beginning of a immunostaining experiment.		*PBST (Triton) is for example used for washing in immunohistology. A 1% PBS Triton X-100 wash can also be added to fixed tissue sections for 10 minutes (followed by 3 time, 15-minute PBS wash) to permeabilize tissue prior to blocking at the beginning of a immunostaining experiment.

Torsten Waldminghaus at 14:40, 12 January 2009

Torsten Waldminghaus — Mon, 12 Jan 2009 14:40:59 GMT

←Older revision		Revision as of 14:40, 12 January 2009
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	==Purpose==		==Purpose==
-	* [[PBS]] stands for phosphate buffered saline. Adding of Tween 20 or TritonX100 makes it PBST (people use the name PBST for both).	+	* [[PBS]] stands for phosphate buffered saline. Adding of the detergent Tween 20 or TritonX100 makes it PBST (people use the name PBST for both).
		+	*PBST (Triton) is for example used for washing in immunohistology. A 1% PBS Triton X-100 wash can also be added to fixed tissue sections for 10 minutes (followed by 3 time, 15-minute PBS wash) to permeabilize tissue prior to blocking at the beginning of a immunostaining experiment.

	==Recipe==		==Recipe==

Torsten Waldminghaus at 14:35, 12 January 2009

Torsten Waldminghaus — Mon, 12 Jan 2009 14:35:10 GMT

←Older revision		Revision as of 14:35, 12 January 2009
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	==Purpose==		==Purpose==
-	* [[PBS]] stands for phosphate buffered saline. Adding of Tween makes it PBST ~~that is used~~ for ~~washing~~.	+	* [[PBS]] stands for phosphate buffered saline. Adding of Tween 20 or TritonX100 makes it PBST (people use the name PBST for both).

	==Recipe==		==Recipe==

Torsten Waldminghaus: New page: ==Purpose== * PBS stands for phosphate buffered saline. Adding of Tween makes it PBST that is used for washing. ==Recipe== *See PBS Category:Material Category:Buffers

Torsten Waldminghaus — Fri, 12 Dec 2008 09:47:06 GMT

New page: ==Purpose== * PBS stands for phosphate buffered saline. Adding of Tween makes it PBST that is used for washing. ==Recipe== *See PBS Category:Material Category:Buffers

New page

==Purpose==
* [[PBS]] stands for phosphate buffered saline. Adding of Tween makes it PBST that is used for washing.

==Recipe==
*See [[PBS]]

[[Category:Material]] [[Category:Buffers]]