PBST: Difference between revisions
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==Purpose== | ==Purpose== | ||
* [[PBS]] stands for phosphate buffered saline. Adding of the detergent Tween 20 or | * [[PBS]] stands for phosphate buffered saline. Adding of the detergent Tween 20 or Triton X-100 makes it PBST (people use the name PBST for both). | ||
*PBST (Triton) is for example used for washing in immunohistology. A 1% PBS Triton X-100 wash can also be added to fixed tissue sections for 10 minutes (followed by 3 time, 15-minute PBS wash) to permeabilize tissue prior to blocking at the beginning of a immunostaining experiment. | *PBST (Triton) is for example used for washing in immunohistology. A 1% PBS Triton X-100 wash can also be added to fixed tissue sections for 10 minutes (followed by 3 time, 15-minute PBS wash) to permeabilize tissue prior to blocking at the beginning of a immunostaining experiment. | ||
Revision as of 07:41, 12 January 2009
Purpose
- PBS stands for phosphate buffered saline. Adding of the detergent Tween 20 or Triton X-100 makes it PBST (people use the name PBST for both).
- PBST (Triton) is for example used for washing in immunohistology. A 1% PBS Triton X-100 wash can also be added to fixed tissue sections for 10 minutes (followed by 3 time, 15-minute PBS wash) to permeabilize tissue prior to blocking at the beginning of a immunostaining experiment.
Recipe
- See PBS
