PBST: Difference between revisions

Latest revision as of 18:42, 23 December 2012

PBS stands for phosphate buffered saline. Adding of the detergent Tween 20 or Triton X-100 makes it PBST (people use the name PBST/PBS-T for both).

PBST (Triton) is for example used for washing in immunohistology. A 1% PBS Triton X-100 wash can also be added to fixed tissue sections for 10 minutes followed by 3 x 15 minutes PBS wash to permeabilize tissue prior to blocking at the beginning of a immunostaining experiment.
PBST is used in protein blots for washing of membrane labelled with antibody.

0.1% (v/v) Tween 20 in 1x PBS

examples

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-==Purpose==
+[[PBS]] stands for phosphate buffered saline. Adding of the detergent Tween 20 or Triton X-100 makes it PBST (people use the name PBST/PBS-T for both).
-* [[PBS]] stands for phosphate buffered saline. Adding of the detergent Tween 20 or Triton X-100 makes it PBST (people use the name PBST for both).
-*PBST (Triton) is for example used for washing in immunohistology. A 1% PBS Triton X-100 wash can also be added to fixed tissue sections for 10 minutes (followed by 3 time, 15-minute PBS wash) to permeabilize tissue prior to blocking at the beginning of a immunostaining experiment.
-==Recipe==
+== Purpose ==
-*See [[PBS]]
+* PBST (Triton) is for example used for washing in immunohistology. A 1% PBS Triton X-100 wash can also be added to fixed tissue sections for 10 minutes followed by 3 x 15 minutes PBS wash to permeabilize tissue prior to blocking at the beginning of a immunostaining experiment.
+* PBST is used in [[protein blots]] for washing of membrane labelled with antibody.
+== Recipe ==
+.1% (v/v) Tween 20 in 1x [[PBS]]
+examples
+* 100µl in 100ml 1x PBS
+* 1ml in 1L 1xPBS
 [[Category:Material]] [[Category:Buffers]]