PBST: Difference between revisions
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(New page: ==Purpose== * PBS stands for phosphate buffered saline. Adding of Tween makes it PBST that is used for washing. ==Recipe== *See PBS Category:Material Category:Buffers) |
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[[PBS]] stands for phosphate buffered saline. Adding of the detergent Tween 20 or Triton X-100 makes it PBST (people use the name PBST/PBS-T for both). | |||
==Recipe== | == Purpose == | ||
* PBST (Triton) is for example used for washing in immunohistology. A 1% PBS Triton X-100 wash can also be added to fixed tissue sections for 10 minutes (followed by 3 time, 15-minute PBS wash) to permeabilize tissue prior to blocking at the beginning of a immunostaining experiment. | |||
* PBST is used in [[protein blots]] for washing of membrane labelled with antibody. | |||
== Recipe == | |||
0.1% (v/v) Tween 20 in 1x [[PBS]] | |||
examples | |||
* 100µl in 100ml 1x PBS | |||
* 1ml in 1L 1xPBS | |||
[[Category:Material]] [[Category:Buffers]] | [[Category:Material]] [[Category:Buffers]] | ||
Revision as of 06:15, 5 May 2009
PBS stands for phosphate buffered saline. Adding of the detergent Tween 20 or Triton X-100 makes it PBST (people use the name PBST/PBS-T for both).
Purpose
- PBST (Triton) is for example used for washing in immunohistology. A 1% PBS Triton X-100 wash can also be added to fixed tissue sections for 10 minutes (followed by 3 time, 15-minute PBS wash) to permeabilize tissue prior to blocking at the beginning of a immunostaining experiment.
- PBST is used in protein blots for washing of membrane labelled with antibody.
Recipe
0.1% (v/v) Tween 20 in 1x PBS
examples
- 100µl in 100ml 1x PBS
- 1ml in 1L 1xPBS
