PBS - Revision history

Nessie Van Loan: /* Recipe */ added dibasic heptahydrate

Nessie Van Loan — Wed, 22 Apr 2009 15:22:25 GMT

Recipe: added dibasic heptahydrate

←Older revision		Revision as of 15:22, 22 April 2009
Line 9:		Line 9:
	#* 80 g NaCl		#* 80 g NaCl
	#* 2 g KCl		#* 2 g KCl
-	#* 14.4 g Na<sub>2</sub>HPO<sub>4</sub> (dibasic) or 18.1 g Na<sub>2</sub>HPO<sub>4</sub> · 2H<sub>2</sub>O	+	#* 14.4 g Na<sub>2</sub>HPO<sub>4</sub> (dibasic anhydrous) OR 18.1 g Na<sub>2</sub>HPO<sub>4</sub> · 2H<sub>2</sub>O (dibasic dihydrate) OR 27.2 g Na<sub>2</sub>HPO<sub>4</sub> · 7H<sub>2</sub>O (dibasic heptahydrate)
-	#* 2.4 g KH<sub>2</sub>PO<sub>4</sub> (monobasic)	+	#* 2.4 g KH<sub>2</sub>PO<sub>4</sub> (monobasic anhydrous)
	#* 800 mL distilled H<sub>2</sub>O		#* 800 mL distilled H<sub>2</sub>O
	# Adjust pH to 7.4 with HCl		# Adjust pH to 7.4 with HCl

Torsten Waldminghaus — Wed, 22 Apr 2009 13:02:14 GMT

Recipe

←Older revision		Revision as of 13:02, 22 April 2009
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	==Recipe==		==Recipe==
-	1x PBS is 137 mM NaCl, 10 mM Phosphate, 2.7 mM KCl, pH 7.4	+	1x PBS is 137 mM NaCl, 12 mM Phosphate, 2.7 mM KCl, pH 7.4

	To make 10x PBS,		To make 10x PBS,
Line 9:		Line 9:
	#* 80 g NaCl		#* 80 g NaCl
	#* 2 g KCl		#* 2 g KCl
-	#* 14.4 g Na<sub>2</sub>HPO<sub>4</sub> (dibasic)	+	#* 14.4 g Na<sub>2</sub>HPO<sub>4</sub> (dibasic) or 18.1 g Na<sub>2</sub>HPO<sub>4</sub> · 2H<sub>2</sub>O
	#* 2.4 g KH<sub>2</sub>PO<sub>4</sub> (monobasic)		#* 2.4 g KH<sub>2</sub>PO<sub>4</sub> (monobasic)
	#* 800 mL distilled H<sub>2</sub>O		#* 800 mL distilled H<sub>2</sub>O

Nessie Van Loan — Tue, 21 Apr 2009 23:49:39 GMT

Nessie Van Loan — Tue, 21 Apr 2009 23:47:43 GMT

Specified monobasic/dibasic for phospates

←Older revision		Revision as of 23:47, 21 April 2009
Line 9:		Line 9:
	#* 80 g NaCl		#* 80 g NaCl
	#* 2 g KCl		#* 2 g KCl
-	#* 14.4 g Na<sub>2</sub>HPO<sub>4</sub>	+	#* 14.4 g Na<sub>2</sub>HPO<sub>4</sub> (dibasic)
-	#* 2.4 g KH<sub>2</sub>PO<sub>4</sub>	+	#* 2.4 g KH<sub>2</sub>PO<sub>4</sub> (monobasic)
	#* 800 mL distilled H<sub>2</sub>O		#* 800 mL distilled H<sub>2</sub>O
	# Adjust pH to 7.4 with HCl		# Adjust pH to 7.4 with HCl

Torsten Waldminghaus — Mon, 12 Jan 2009 14:38:49 GMT

Recipe: moved parts to PBST

←Older revision		Revision as of 14:38, 12 January 2009
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	*'''[[PBST]]''': For some applications (washing) one adds Tween 20 PBS which makes [[PBST]]		*'''[[PBST]]''': For some applications (washing) one adds Tween 20 PBS which makes [[PBST]]
-	**Usual concentrations of Tween 20 in ~~TBST~~ are 0.1 or 0.05%.	+	**Usual concentrations of Tween 20 in PBST are 0.1 or 0.05%.
-		+	**Usual concentration of Triton X-100 in TBST is 0.1-0.3%.
-	~~Another buffer used for washing~~ in ~~immunohistology~~ is ~~PBS Triton, which contains~~ 0.1%-0.3% of the detergent Triton X-100. A 1% PBS Triton X-100 wash can also be added to fixed tissue sections for 10 minutes (followed by 3 time, 15-minute PBS wash) to permeabilize tissue prior to blocking at the beginning of a immunostaining experiment.	+

	==Other Links==		==Other Links==

Ryan T. Willett — Wed, 07 Jan 2009 20:51:03 GMT

Recipe

←Older revision		Revision as of 20:51, 7 January 2009
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	**Usual concentrations of Tween 20 in TBST are 0.1 or 0.05%.		**Usual concentrations of Tween 20 in TBST are 0.1 or 0.05%.

-	Another buffer used for washing in immunohistology is PBS Triton, which contains 0.1%-0.3% of the detergent Triton X-100. A 1% Triton X-100 can also be added to fixed tissue sections for 10 minutes (followed by 3 time, 15-minute PBS wash) to permeabilize tissue prior to blocking at the beginning of a immunostaining experiment.	+	Another buffer used for washing in immunohistology is PBS Triton, which contains 0.1%-0.3% of the detergent Triton X-100. A 1% PBS Triton X-100 wash can also be added to fixed tissue sections for 10 minutes (followed by 3 time, 15-minute PBS wash) to permeabilize tissue prior to blocking at the beginning of a immunostaining experiment.

	==Other Links==		==Other Links==

Ryan T. Willett — Wed, 07 Jan 2009 20:50:26 GMT

Recipe

←Older revision		Revision as of 20:50, 7 January 2009
Line 20:		Line 20:
	**Usual concentrations of Tween 20 in TBST are 0.1 or 0.05%.		**Usual concentrations of Tween 20 in TBST are 0.1 or 0.05%.

		+	Another buffer used for washing in immunohistology is PBS Triton, which contains 0.1%-0.3% of the detergent Triton X-100. A 1% Triton X-100 can also be added to fixed tissue sections for 10 minutes (followed by 3 time, 15-minute PBS wash) to permeabilize tissue prior to blocking at the beginning of a immunostaining experiment.

	==Other Links==		==Other Links==

Torsten Waldminghaus — Fri, 12 Dec 2008 09:49:43 GMT

←Older revision		Revision as of 09:49, 12 December 2008
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	# Add H<sub>2</sub>O to 1L		# Add H<sub>2</sub>O to 1L
	# Autoclave for 20 minutes on liquid cycle. Store at RT.		# Autoclave for 20 minutes on liquid cycle. Store at RT.
		+
		+
		+	*'''[[PBST]]''': For some applications (washing) one adds Tween 20 PBS which makes [[PBST]]
		+	**Usual concentrations of Tween 20 in TBST are 0.1 or 0.05%.
		+

	==Other Links==		==Other Links==

Torsten Waldminghaus — Wed, 07 May 2008 13:49:16 GMT

←Older revision		Revision as of 13:49, 7 May 2008
Line 1:		Line 1:
		+	==Purpose==
	PBS stands for phosphate buffered saline and is often used because it is isotonic to cells.		PBS stands for phosphate buffered saline and is often used because it is isotonic to cells.

		+	==Recipe==
	1x PBS is 137 mM NaCl, 10 mM Phosphate, 2.7 mM KCl, pH 7.4		1x PBS is 137 mM NaCl, 10 mM Phosphate, 2.7 mM KCl, pH 7.4

Line 7:		Line 9:
	#* 80 g NaCl		#* 80 g NaCl
	#* 2 g KCl		#* 2 g KCl
-	#* 14.4 g ~~Na2HPO4~~	+	#* 14.4 g Na<sub>2</sub>HPO<sub>4</sub>
-	#* 2.4 g ~~KH2PO4~~	+	#* 2.4 g KH<sub>2</sub>PO<sub>4</sub>
-	#* 800 mL distilled ~~H2O~~	+	#* 800 mL distilled H<sub>2</sub>O
	# Adjust pH to 7.4 with HCl		# Adjust pH to 7.4 with HCl
-	# Add ~~H2O~~ to 1L	+	# Add H<sub>2</sub>O to 1L
	# Autoclave for 20 minutes on liquid cycle. Store at RT.		# Autoclave for 20 minutes on liquid cycle. Store at RT.

Torsten Waldminghaus — Tue, 11 Mar 2008 21:33:59 GMT

added tags

←Older revision		Revision as of 21:33, 11 March 2008
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	* [[Silver: 10x PBS]]		* [[Silver: 10x PBS]]
	* [http://en.wikipedia.org/wiki/Phosphate_buffered_saline wikipedia article]		* [http://en.wikipedia.org/wiki/Phosphate_buffered_saline wikipedia article]
		+
		+	[[Category:Material]] and [[Category:Buffers]]

←Older revision		Revision as of 23:49, 21 April 2009
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	* [http://en.wikipedia.org/wiki/Phosphate_buffered_saline wikipedia article]		* [http://en.wikipedia.org/wiki/Phosphate_buffered_saline wikipedia article]

-	[[Category:Material]] ~~and~~ [[Category:Buffers]]	+	[[Category:Material]] [[Category:Buffers]]