PBS: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 2: | Line 2: | ||
80 g NaCl | 80 g NaCl | ||
2 g KCl | 2 g KCl | ||
14.4 g Na2HPO4 | 14.4 g Na2HPO4 | ||
2.4 g KH2PO4 | 2.4 g KH2PO4 | ||
800 mL distilled H2O | 800 mL distilled H2O | ||
Revision as of 15:44, 14 June 2005
1. Combine the following:
80 g NaCl
2 g KCl
14.4 g Na2HPO4
2.4 g KH2PO4
800 mL distilled H2O
2. Adjust pH to 7.4 with HCl
3. Add H2O to 1L
4. Autoclave for 20 minutes on liquid cycle. Store at RT.
