PBS: Difference between revisions
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800 mL distilled H2O | 800 mL distilled H2O | ||
2. Adjust pH to 7.4 with HCl | 2. Adjust pH to 7.4 with HCl | ||
3. Add H2O to 1L | 3. Add H2O to 1L | ||
4. Autoclave for 20 minutes on liquid cycle. Store at RT. | 4. Autoclave for 20 minutes on liquid cycle. Store at RT. | ||
Revision as of 15:45, 14 June 2005
1. Combine the following:
80 g NaCl
2 g KCl
14.4 g Na2HPO4
2.4 g KH2PO4
800 mL distilled H2O
2. Adjust pH to 7.4 with HCl
3. Add H2O to 1L
4. Autoclave for 20 minutes on liquid cycle. Store at RT.
