Our Approach and Goals

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Current revision (23:45, 26 October 2013) (view source)
 
Line 1: Line 1:
<Font Size="4">
<Font Size="4">
-
<table border="1" cellpadding="5" width="900" >
+
<table cellspacing="7" cellpadding="10" width="920" >
<tr align="center">
<tr align="center">
-
<td width="180" bgcolor="#002233">[[Biomod/2013/Fukuoka|<font face="Comic Sans MS,cursive,Arial" color="white">Top</font>]] </td>
+
<td width="180" bgcolor="#002233">[[Biomod/2013/Fukuoka|<font face="cursive,fantasy,Arial" color="white">Top</font>]] </td>
-
<td width="180" bgcolor="#002233">[[Introduction|<font face="Comic Sans MS,cursive,Arial" color="white">Introduction</font>]] </td>
+
<td width="180" bgcolor="#002233">[[Introduction|<font face="cursive,fantasy,Arial" color="white">Introduction</font>]] </td>
-
<td width="180" bgcolor="#002233">[[Our Approach and Goals|<font face="Comic Sans MS,cursive,Arial" color="white">Approach and Goals</font>]] </td>
+
<td width="180" bgcolor="#002233">[[Our Approach and Goals|<font face="cursive,fantasy,Arial" color="white">Approach and Goals</font>]] </td>
-
<td width="180" bgcolor="#002233">[[Method|<font face="Comic Sans MS,cursive,Arial" color="white">Method</font>]] </td>
+
<td width="180" bgcolor="#002233">[[Method|<font face="cursive,fantasy,Arial" color="white">Method</font>]] </td>
-
<td width="180" bgcolor="#002233">[[Results and Discussion|<font face="Comic Sans MS,cursive,Arial" color="white">Results and Discussion</font>]] </td>
+
<td width="180" bgcolor="#002233">[[Results and Discussion|<font face="cursive,fantasy,Arial" color="white">Results and Discussion</font>]] </td>
-
<td width="180" bgcolor="#002233">[[Member|<font face="Comic Sans MS,cursive,Arial" color="white">Member</font>]] </td>
+
<td width="180" bgcolor="#002233">[[Member|<font face="cursive,fantasy,Arial" color="white">Member</font>]] </td>
-
<td width="180" bgcolor="#002233">[[Sponsor|<font face="Comic Sans MS,cursive,Arial" color="white">Sponsor</font>]] </td>
+
<td width="180" bgcolor="#002233">[[Sponsor|<font face="cursive,fantasy" color="white">Sponsor</font>]] </td>
</table>
</table>
 +
*As the start point to solve the problems, we here design the simplest nano-device that rotates: the weathercock. We fabricate the rotating weathercock to detect water flow by combining inorganic nanoporous substrate with the nanoscale weathercock made by DNA origami. (fig.2-1)
*As the start point to solve the problems, we here design the simplest nano-device that rotates: the weathercock. We fabricate the rotating weathercock to detect water flow by combining inorganic nanoporous substrate with the nanoscale weathercock made by DNA origami. (fig.2-1)

Current revision


Top Introduction Approach and Goals Method Results and Discussion Member Sponsor


  • As the start point to solve the problems, we here design the simplest nano-device that rotates: the weathercock. We fabricate the rotating weathercock to detect water flow by combining inorganic nanoporous substrate with the nanoscale weathercock made by DNA origami. (fig.2-1)
fig.2-1
fig.2-1


  • We synthesize the DNA-origami-based “DNA weathercock” consist of the blade part which sense the water flow (instead of the wind in air) and the shaft part. (fig.2-2) The shaft is mounted into the vertical nanopores of "anodizing alumina" which is synthesized by electrode reaction of an aluminiuma metal plate. (fig.2-3) The size of the nanopore is tuned by changing the synthetic condition of the nanoporous alumina in the range of 20 nm - microns. We can further optimize the interaction between the DNA shaft and alumina surface by chemical modification of the alumina surface.


fig.2-2
fig.2-2
fig.2-3
fig.2-3






  • When the DNA weathercock catches the flow in the fluid, it turns to the direction of the flow. Since the head of the weathercock is attached with a fluorescence molecule, the flow direction is detected by the observation by confocal laser microsope or even by our naked-eyes through a polarizing film. (fig.2-4)
fig.2-4
fig.2-4


  • The criteria for the success of this project is as follows:
    • The synthesis and characterization of the nanoporous substrate
    • The design, synthesis and characterization of DNA weathercock
    • Mounting the DNA weathercock to the porous substrate
    • Observation of the rotation of the weathercock induced by flow