Our Approach and Goals: Difference between revisions
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<td width="180" bgcolor="#002233">[[Biomod/2013/Fukuoka|<font face=" | <td width="180" bgcolor="#002233">[[Biomod/2013/Fukuoka|<font face="cursive,fantasy,Arial" color="white">Top</font>]] </td> | ||
<td width="180" bgcolor="#002233">[[Introduction|<font face=" | <td width="180" bgcolor="#002233">[[Introduction|<font face="cursive,fantasy,Arial" color="white">Introduction</font>]] </td> | ||
<td width="180" bgcolor="#002233">[[Our Approach and Goals|<font face=" | <td width="180" bgcolor="#002233">[[Our Approach and Goals|<font face="cursive,fantasy,Arial" color="white">Approach and Goals</font>]] </td> | ||
<td width="180" bgcolor="#002233">[[Method|<font face=" | <td width="180" bgcolor="#002233">[[Method|<font face="cursive,fantasy,Arial" color="white">Method</font>]] </td> | ||
<td width="180" bgcolor="#002233">[[Results and Discussion|<font face=" | <td width="180" bgcolor="#002233">[[Results and Discussion|<font face="cursive,fantasy,Arial" color="white">Results and Discussion</font>]] </td> | ||
<td width="180" bgcolor="#002233">[[Member|<font face=" | <td width="180" bgcolor="#002233">[[Member|<font face="cursive,fantasy,Arial" color="white">Member</font>]] </td> | ||
<td width="180" bgcolor="#002233">[[Sponsor|<font face=" | <td width="180" bgcolor="#002233">[[Sponsor|<font face="cursive,fantasy" color="white">Sponsor</font>]] </td> | ||
</table> | </table> | ||
*As the start point to solve the problems, we here design the simplest nano-device that rotates: the weathercock. We fabricate the rotating weathercock to detect water flow by combining inorganic nanoporous substrate with the nanoscale weathercock made by DNA origami. (fig.2-1) | |||
[[Image:Fit2013 13.png|380px|center|thumb|fig.2-1]] | |||
*We synthesize the DNA-origami-based “DNA weathercock” consist of the blade part which sense the water flow (instead of the wind in air) and the shaft part. (fig.2-2) The shaft is mounted into the vertical nanopores of "anodizing alumina" which is synthesized by electrode reaction of an aluminiuma metal plate. (fig.2-3) The size of the nanopore is tuned by changing the synthetic condition of the nanoporous alumina in the range of 20 nm - microns. We can further optimize the interaction between the DNA shaft and alumina surface by chemical modification of the alumina surface. | |||
[[Image:Weathercock.png|360px|left|thumb|fig.2-2]][[Image:Fit2013 12.png|450px|center|thumb|fig.2-3]] | |||
<br> | |||
*When the DNA weathercock catches the flow in the fluid, it turns to the direction of the flow. Since the head of the weathercock is attached with a fluorescence molecule, the flow direction is detected by the observation by confocal laser microsope or even by our naked-eyes through a polarizing film. (fig.2-4) | |||
[[Image:Fit2013 05.png|380px|center|thumb|fig.2-4]] | |||
<br> | |||
*The criteria for the success of this project is as follows: | |||
**The synthesis and characterization of the nanoporous substrate | |||
**The design, synthesis and characterization of DNA weathercock | |||
**Mounting the DNA weathercock to the porous substrate | |||
**Observation of the rotation of the weathercock induced by flow | |||
Revision as of 20:45, 26 October 2013
| Top | Introduction | Approach and Goals | Method | Results and Discussion | Member | Sponsor |
- As the start point to solve the problems, we here design the simplest nano-device that rotates: the weathercock. We fabricate the rotating weathercock to detect water flow by combining inorganic nanoporous substrate with the nanoscale weathercock made by DNA origami. (fig.2-1)
- We synthesize the DNA-origami-based “DNA weathercock” consist of the blade part which sense the water flow (instead of the wind in air) and the shaft part. (fig.2-2) The shaft is mounted into the vertical nanopores of "anodizing alumina" which is synthesized by electrode reaction of an aluminiuma metal plate. (fig.2-3) The size of the nanopore is tuned by changing the synthetic condition of the nanoporous alumina in the range of 20 nm - microns. We can further optimize the interaction between the DNA shaft and alumina surface by chemical modification of the alumina surface.
- When the DNA weathercock catches the flow in the fluid, it turns to the direction of the flow. Since the head of the weathercock is attached with a fluorescence molecule, the flow direction is detected by the observation by confocal laser microsope or even by our naked-eyes through a polarizing film. (fig.2-4)
- The criteria for the success of this project is as follows:
- The synthesis and characterization of the nanoporous substrate
- The design, synthesis and characterization of DNA weathercock
- Mounting the DNA weathercock to the porous substrate
- Observation of the rotation of the weathercock induced by flow
