Others 10. 박재형(JaeHyung Park): Difference between revisions

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[http://pubs.acs.org/doi/abs/10.1021/ja4008003 link title]
[http://pubs.acs.org/doi/abs/10.1021/ja4008003 link title]
*정확한 길이의 빌딩블록을 자가조립하는 것은 쉽지않다.
*정확한 길이의 빌딩블록을 자가조립하는 것은 쉽지않다.
:
:섬유형 바이러스의 모양을 모방하여 정밀하게 길이를 제어할 수 있다.




Revision as of 08:22, 1 February 2015

Paper Study

2. 1 (Sun)

Precision Templating with DNA of a Virus-like Particle with Peptide Nanostructures

http://pubs.acs.org/doi/abs/10.1021/ja4008003#close Box file name : Ruff2013J_Am_Chem_Soc(jae hyung park).pdf
link title

  • 정확한 길이의 빌딩블록을 자가조립하는 것은 쉽지않다.
섬유형 바이러스의 모양을 모방하여 정밀하게 길이를 제어할 수 있다.



caDNAno

1. 22 (Thu)




Paper Study

1. 20 (Tue)

End-joining long nucleic acid polymers - STV

http://nar.oxfordjournals.org/content/36/16/e104.short#close Box file name : Van_den_Hout2008Nucleic_Acids_Res(Jaehyung Park).pdf, PJH150120_stv
link title

  • Ligation

DNA나 RNA의 끝을 이어주는 과정 single-strand, long-nucleic acid일 경우 효율이 떨어지는 단점

  • Biotin–streptavidin linkage

streptavidin은 단백질의 일종 biotin은 비타민의 일종으로 강한 결합력으로 결합함.
강한 결합력을 이용해 end-joining을 하는 방법.
2-step으로 이루어짐 한분자에 stv를 붙이고(1-step)
unbound stv를 제거한 다음 두번째 분자를 붙임(2-step)

  • Gel electrophoresis

전극을 이용하여 물질을 이동시키며 크기나 구조에 따라 이동속도의 차이가 일어남.
크기를 분석하는 방법.

  • The application of Biotin–streptavidin linkage

single-strand를 필요로 하는 실험에 쓰임.
강한 결합력을 이용하여 병원성 박테리아의 선택적 포획 등에도 쓰임.

Paper Study

1. 3 (Sat)

Folding DNA to create nanoscale shapes and patterns - DNA origami

http://www.nature.com/nature/journal/v440/n7082/full/nature04586.html#close Box file name : Nature04586(PJH).pdf, PJH150103_dna origami.pptx
link title

  • DNA Structure
  1. DNA is double helix structure.
  2. In DNA, there are four different types of nitrogenous base.
    According to base pairing rules (A with T and C with G), hydrogen bonds bind the nitrogenous bases of the two separate polynucleotide strands to make double-stranded DNA.
    • A is for adenine
    • G is for guanine
    • C is for cytosine
    • T is for thymine
  • DNA origami
  1. DNA origami is the nanoscale folding of DNA to create arbitrary two- and three-dimensional shapes at the nanoscale.
  2. A long single strand DNA and staple DNA strand are required
    • The use of a long single strand DNA in M13mp18.
    • Staple DNA strand folded long single strand DNA.
    • Use a computer to determine the way to create the correct staples needed to form a certain shape. And we create arbitrary two- and three-dimensional shapes at the nanoscale.

  • The application of DNA origami
  1. DNA origami will enable making small computer.
  2. DNA origami be used to create nanorobots capable of finding and destroying cancer cells in the human body.
  • Consideration
DNA origami was very impressive and DNA origami technology development will continue.
So DNA origami can be adapted to create more complex or larger structures.
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