Others 10. 박재형(JaeHyung Park): Difference between revisions
No edit summary |
No edit summary |
||
Line 5: | Line 5: | ||
[http://pubs.acs.org/doi/abs/10.1021/ja4008003 link title] | [http://pubs.acs.org/doi/abs/10.1021/ja4008003 link title] | ||
*정확한 길이의 빌딩블록을 자가조립하는 것은 쉽지않다. | *정확한 길이의 빌딩블록을 자가조립하는 것은 쉽지않다. | ||
: | :섬유형 바이러스의 모양을 모방하여 정밀하게 길이를 제어할 수 있다. | ||
Revision as of 08:22, 1 February 2015
Paper Study
2. 1 (Sun)
Precision Templating with DNA of a Virus-like Particle with Peptide Nanostructures
http://pubs.acs.org/doi/abs/10.1021/ja4008003#close Box file name : Ruff2013J_Am_Chem_Soc(jae hyung park).pdf
link title
- 정확한 길이의 빌딩블록을 자가조립하는 것은 쉽지않다.
- 섬유형 바이러스의 모양을 모방하여 정밀하게 길이를 제어할 수 있다.
caDNAno
1. 22 (Thu)
Paper Study
1. 20 (Tue)
End-joining long nucleic acid polymers - STV
http://nar.oxfordjournals.org/content/36/16/e104.short#close Box file name : Van_den_Hout2008Nucleic_Acids_Res(Jaehyung Park).pdf, PJH150120_stv
link title
- Ligation
DNA나 RNA의 끝을 이어주는 과정 single-strand, long-nucleic acid일 경우 효율이 떨어지는 단점
- Biotin–streptavidin linkage
streptavidin은 단백질의 일종 biotin은 비타민의 일종으로
강한 결합력으로 결합함.
강한 결합력을 이용해 end-joining을 하는 방법.
2-step으로 이루어짐 한분자에 stv를 붙이고(1-step)
unbound stv를 제거한 다음 두번째 분자를 붙임(2-step)
- Gel electrophoresis
전극을 이용하여 물질을 이동시키며
크기나 구조에 따라 이동속도의 차이가 일어남.
크기를 분석하는 방법.
- The application of Biotin–streptavidin linkage
single-strand를 필요로 하는 실험에 쓰임.
강한 결합력을 이용하여 병원성 박테리아의 선택적 포획 등에도 쓰임.
Paper Study
1. 3 (Sat)
Folding DNA to create nanoscale shapes and patterns - DNA origami
http://www.nature.com/nature/journal/v440/n7082/full/nature04586.html#close Box file name : Nature04586(PJH).pdf, PJH150103_dna origami.pptx
link title
- DNA Structure
- DNA is double helix structure.
- In DNA, there are four different types of nitrogenous base.
According to base pairing rules (A with T and C with G), hydrogen bonds bind the nitrogenous bases of the two separate polynucleotide strands to make double-stranded DNA.- A is for adenine
- G is for guanine
- C is for cytosine
- T is for thymine
- DNA origami
- DNA origami is the nanoscale folding of DNA to create arbitrary two- and three-dimensional shapes at the nanoscale.
- A long single strand DNA and staple DNA strand are required
- The use of a long single strand DNA in M13mp18.
- Staple DNA strand folded long single strand DNA.
- Use a computer to determine the way to create the correct staples needed to form a certain shape. And we create arbitrary two- and three-dimensional shapes at the nanoscale.
- The application of DNA origami
- DNA origami will enable making small computer.
- DNA origami be used to create nanorobots capable of finding and destroying cancer cells in the human body.
- Consideration
- DNA origami was very impressive and DNA origami technology development will continue.
So DNA origami can be adapted to create more complex or larger structures.