OpenWetWare/Page Updates/Raw Data/2005

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2005

04/2005

Common: 165
Groups: 10
IGEM: 0
Protocols: 72
Materials: 24
Labs: 27

Details

Group Pages

(10) Synthetic Biology

Lab Pages

(26) Endy Lab
(1) Knight

IGEM Pages

Protocol Pages

(35) Protocols
(8) MIT:Sequencing BioBrick DNA
(8) High Efficiency Transformation
(4) Electrocompetent cells
(4) RNA extraction
(3) DNA ligation
(2) SortoStat/Operation
(2) Preparing chemically competent cells
(1) Yeast Colony PCR
(1) Yeast DNA Prep
(1) Design a Microfluidic Chip
(1) Agarose gel electrophoresis
(1) Endy:Pouring plates
(1) Agarose Pads for Microscopy

Material Pages

Common Pages

05/2005

Common: 147
Groups: 13
IGEM: 93
Protocols: 107
Materials: 30
Labs: 38

Details

Group Pages

(7) Synthetic Biology
(6) BE Board

Lab Pages

(36) Endy Lab
(2) Knight

IGEM Pages

(93) IGEM

Protocol Pages

(15) Protocols
(11) Order a Microfluidic Chip from the Foundry
(10) SortoStat/Operation
(8) Annealing primers
(7) Designing primers
(7) DNA ligation
(6) Miniprep
(6) Agarose gel electrophoresis
(5) PNK Treatment of DNA Ends
(5) Endy:Pouring plates
(4) Purification of DNA
(4) Preparing chemically competent cells
(3) Design a Microfluidic Chip
(3) Electroporation
(3) Ethanol precipitation of small DNA fragments
(2) Knight:Micropure EZ and Microcon purification
(2) Cell Cycle Arrest
(2) Endy:Templiphi
(1) MIT:Sequencing BioBrick DNA
(1) Phosphatase treatment of linearized vector
(1) Electrocompetent cells
(1) High Efficiency Transformation

Material Pages

Common Pages

06/2005

Common: 618
Groups: 36
IGEM: 1255
Protocols: 102
Materials: 59
Labs: 299

Details

Group Pages

(13) Synthetic Biology
(10) BEInG
(7) BE Board
(5) Bbf
(1) Seminar

Lab Pages

(186) Endy Lab
(56) Grossman Lab
(25) Knight
(24) Grossman Lab
(8) Endy Lab

IGEM Pages

(1255) IGEM

Protocol Pages

(18) Protocols
(15) In vitro transcription with T7 RNA polymerase
(12) DNA ligation
(9) SortoStat/Operation
(5) Studier Lysate Prep
(5) Preparing chemically competent cells
(5) MIT:Sequencing BioBrick DNA
(4) Cesium Chloride Purification of T7
(3) Questions and Answers
(3) Agarose gel electrophoresis
(3) Knight:DNA ligation using NEB Quick Ligation Kit
(3) Knight:Annealing and primer extension with Klenow polymerase
(2) Pulse-chase protein production
(2) Colony PCR
(2) Annealing primers
(1) Knight:Micropure EZ and Microcon purification
(1) Modeller
(1) Knight:Colony PCR
(1) Beta-galactosidase assay
(1) Fixing cells
(1) Electrocompetent cells
(1) RNA extraction
(1) Order a Microfluidic Chip from the Foundry
(1) High Efficiency Transformation
(1) Design a Microfluidic Chip
(1) Phosphatase treatment of linearized vector

Material Pages

(14) Materials
(6) LB
(5) Kanamycin
(4) Raffinose
(3) Neidhardt EZ Rich Defined
(3) PBS
(3) CFP
(2) YFP
(2) SC
(2) ONPF
(2) Erythromycin
(2) MRFP1
(1) M9 salts
(1) IPTG
(1) Dyes and stains
(1) DNA ladder
(1) SOC
(1) T broth
(1) TSS
(1) Reverse Transcriptase
(1) Phleomycin
(1) YPD
(1) SD

Common Pages

07/2005

Common: 394
Groups: 57
IGEM: 1379
Protocols: 82
Materials: 14
Labs: 147

Details

Group Pages

Lab Pages

(130) Endy Lab
(9) Endy Lab
(3) Keating
(3) Keating Lab
(1) Grossman Lab
(1) Knight

IGEM Pages

(1379) IGEM

Protocol Pages

(15) Protocols
(8) Site-directed mutagenesis
(7) Bacterial cell culture
(7) Colony PCR
(6) Agarose gel electrophoresis
(5) Preparing chemically competent cells
(5) Restriction digest
(5) Designing primers
(4) DNA ligation
(3) Phenol/chloroform extraction
(3) Endy:STN Chemostat Protocol
(3) Endy:Colony PCR
(3) Endy:E. coli Western Blot
(1) Miniprep
(1) In vitro transcription with T7 RNA polymerase
(1) Electrocompetent cells
(1) Electroporation
(1) Knight:Colony PCR
(1) Yeast DNA Prep
(1) Acrylamide gels
(1) Knight:Restriction Digest

Material Pages

(5) Phusion
(3) Tetracycline
(3) Materials
(2) ATc
(1) Arabinose

Common Pages

08/2005

Common: 596
Groups: 239
IGEM: 548
Protocols: 79
Materials: 7
Labs: 333

Details

Group Pages

Lab Pages

(186) Endy Lab
(50) Sauer Lab
(42) Keating
(26) Keating Lab
(16) Grossman Lab
(7) Knight
(3) Endy Lab
(3) Grossman Lab

IGEM Pages

(548) IGEM

Protocol Pages

(24) Methods to determine the size of an object in microns
(13) Protocols
(6) Sauer:Expression/purification of 35S-Met proteins
(6) Endy:Victor3 plate reader
(4) SortoStat/Operation
(4) Preparing chemically competent cells
(3) Sequencing DNA
(3) Site-directed mutagenesis
(2) RNA extraction
(2) Endy:RNA extraction
(2) Designing primers
(2) Endy:Pouring plates
(2) Agarose gel electrophoresis
(1) Knight:Site-directed mutagenesis/Single site
(1) Electrocompetent cells
(1) Phosphatase treatment of linearized vector
(1) Restriction digest
(1) Endy:Colony PCR
(1) Layered plates

Material Pages

Common Pages

(46) Bioinformatics of S-layer Proteins
(30) BioBricks construction tutorial
(23) Standardized GFP quantification
(19) Error Detection & Correction in Replicating Machines
(18) B-Galactosidase Assay (A better Miller)
(18) Main Page
(16) T7.1
(15) The BioBricks Foundation
(14) T7.2
(14) Equipment
(11) SfGS lecture
(11) Flakey DE Stuff
(11) Sortostat
(10) Coiled Coils that may dimeric or trimeric
(10) SfGS upcoming events (and other ways to get involved)
(9) Hey, cut it out
(9) T7.1/Construction
(9) Studio!Sus
(8) T7.1/Specification
(8) Students for Global Sustainability Wiki
(8) SfGS Midway Sign-up Sheet
(8) SfGS August 26, 2005
(8) Biostuff@mit.edu
(7) Coiled Coils from Paircoils
(7) Sortostat/Optimal sorting cutoffs
(7) OWW BioMicro Server Switch
(7) Other Parameters
(7) Current events
(7) β-Galactosidase Assay (A better Miller)
(6) Who Ordered That?
(6) Coiled Coils
(6) Amino Acid Composition
(6) RWE072605
(6) Studiosus.pdf
(6) Synthetic Biology
(5) Bioreactor Parameters
(5) Theoretical Power generation
(5) 2005 ICSB Conference
(5) Evolutionary Stability
(5) Nikon TE2000 Microscope
(5) Rebuilding T7
(4) July 17 picnic on Cape Cod
(4) Getting started on the microscope
(4) Synthetic Genomics Study
(4) SfGS active members
(4) Reserving a room at MIT
(4) Agarose gel electrophoresis/Common issues
(4) Streak plates
(4) E. coli genotypes
(4) Microfluidic chemostat labview program
(3) Chassis/System - BC Box 1
(3) COMPOSITIONAL ANALYSIS
(3) DNA sequence design to modulate evolutionary stability
(3) Statistical Analysis of Protein Sequence
(3) Device and system design to modulate evolutionary stability
(3) DSpace/Startup
(3) Sortostat/Experiments/Early experiments
(3) LaTeX
(2) Nanodrop
(2) Media formula
(2) Valving, fittings, and tubing
(2) Talks
(2) A Better SDS-PAGE
(2) Endnote 8 to older version of Endnote files
(2) Isadora deese
(2) Extinction coefficients
(2) CHARGE DISTRIBUTIONAL ANALYSIS
(2) SbsB Extinction coefficients
(2) Laser auto-alignment
(2) Writing a letter of recommendation
(2) Beta-Galactosidase Assay (A better Miller)
(2) SfGS planning information
(2) T7.1/Reannotation
(2) Flourless chocolate cake
(2) SfGS publications
(2) Predicts the location of coiled-coil regions in amino acid sequences and classifies the predictions as dimeric or trimeric
(2) SfGS minutes
(2) Sortostat/Setting up a new chip
(2) KM's soapbox
(1) SbsB DNA Sequence
(1) SbsB Amino Acid Composition
(1) Solenoids
(1) Loading dye
(1) Bioreactor Team
(1) SbsB Protein Sequence
(1) Sap Protein Sequence
(1) Strain cells
(1) Cell size
(1) SbpA Amino Acid Composition
(1) Kathleen McGinness
(1) Enzymes
(1) Chemicals
(1) Safety
(1) Light My X,Y,&Z
(1) SbpA Other Parameters
(1) SbpA Extinction coefficients
(1) Yevgenia Khodor
(1) Ykhodor
(1) SbsB Other Parameters
(1) Site-directed Mutagenesis
(1) Genotypes
(1) Tutorials
(1) Absorbance Labels
(1) E. coli Genotypes
(1) Strains
(1) SfGS past events
(1) Biological Energy Interest Group
(1) Sean Moore
(1) Other Resources
(1) Alan Grossman
(1) Sri Kosuri
(1) TABASCO
(1) Registry Wish List
(1) July 2005
(1) June 2005
(1) SAVE Wiki
(1) Device Details
(1) SbpA DNA Sequence
(1) SbpA Protein Sequence
(1) Enzyme selection for BioBrick digest
(1) Restriction Digest
(1) Ania Labno
(1) REPETITIVE STRUCTURES, MULTIPLETS, PERIODICITY ANALYSIS, SPACING ANALYSIS
(1) Protein Sequence
(1) Julie Norville
(1) Flow cell
(1) Pixel size calibration
(1) Electroporator
(1) 5810R Centrifuge
(1) Research
(1) Designing Primers
(1) Alex Mallet

09/2005

Common: 722
Groups: 971
IGEM: 101
Protocols: 57
Materials: 1
Labs: 475

Details

Group Pages

(236) Synthetic Biology
(230) BE Board
(200) Silver
(143) Eigencluster
(70) Sauer
(26) Suggs
(18) Lauffenburger
(15) Matsudaira
(9) Burge
(6) SfGS
(6) Ellington
(3) Tregwiki
(3) KM
(3) SB
(2) Bbf
(1) BE.109

Lab Pages

IGEM Pages

(101) IGEM

Protocol Pages

(8) Protocols
(6) Knight:TOPO TA cloning
(4) Ethanol precipitation of nucleic acids
(4) Site-directed mutagenesis
(3) Silver: Lysate for Western
(3) Acrylamide gels
(3) Knight:Agarose gel electrophoresis
(2) Sauer:Expression/purification of 35S-Met proteins
(2) DNA ligation
(2) PCR
(2) Yeast Colony PCR
(2) Colony PCR
(2) Agarose gel electrophoresis
(2) PNK Treatment of DNA Ends
(1) Silver: Time-Lapse Microscopy
(1) Silver: Colony PCR
(1) Silver: Coomassie Stain
(1) Silver: Destain
(1) Agarose Pads for Microscopy
(1) Sequencing DNA
(1) MIT:Sequencing BioBrick DNA
(1) Studier Lysate Prep
(1) Endy:Victor3 plate reader
(1) Knight:Annealing and primer extension with Klenow polymerase
(1) Restriction digest
(1) Silver: PCR

Material Pages

(1) T broth

Common Pages

10/2005

Common: 352
Groups: 327
IGEM: 118
Protocols: 40
Materials: 0
Labs: 173

Details

Group Pages

(104) BE Board
(45) Eigencluster
(37) Suggs
(33) Synthetic Biology
(31) Silver
(24) Sauer
(18) Matsudaira
(11) Keasling
(9) Other Stuff Re
(7) BE.109
(3) Rao
(2) MIT
(1) SfGS
(1) Wikiomics
(1) Tregwiki

Lab Pages

(110) Endy Lab
(43) Knight
(11) Keating
(4) Keasling Lab
(2) Matsudaira Lab
(1) Rao Lab
(1) Grossman Lab
(1) Suggs Lab

IGEM Pages

(118) IGEM

Protocol Pages

(25) Library Generation
(2) Endy:STN Chemostat Protocol
(2) Protocols
(2) Miniprep
(2) Endy:Victor3 plate reader
(1) Endy:Pouring plates
(1) Pulse-chase protein production
(1) Knight:TOPO TA cloning
(1) Preparing chemically competent cells
(1) Endy:RNA extraction
(1) SortoStat/Operation
(1) Questions and Answers

Material Pages

Common Pages

(40) T7.2/gene specification
(32) Publishing Group
(30) Parts characterization
(28) BE.180
(13) AlexLabNotebook/ChrIIIRebuild/10/1/05-10/7/05
(11) Titratable control of pBAD and lac promoters in individual E. coli cells
(10) Bacterial artificial chromosomes
(10) Synthetic Biology
(9) AlexLabNotebook/ChrIIIRebuild/9/24/05-9/30/05
(9) Main Page
(9) AlexLabNotebook/ChrIIIRebuild
(9) Pam Silver
(7) AlexLabNotebook/Pheromone
(6) MIT Biological Engineering Graduate Student Board
(6) AlexLabNotebook/YeastRebuild
(6) Lauren Ha
(5) TMT Thesis Project
(5) CSBI.100
(5) Publishing Group/Meeting 10-6-05
(4) Codon Optimized for Failure
(4) T7.2
(4) OWW BioMicro Server Switch
(4) Parts characterization/Characterization approaches
(4) Nikon TE2000 Microscope
(4) Equipment
(4) Useful facts
(3) More
(3) Chassis/System - BC Box 1
(3) Ved Sharma
(3) Current events
(3) SfGS lecture
(3) Luis Alvarez
(3) Publishing Group/Meeting 10-4-05
(3) AlexLabNotebook/ChrIIIRebuild/9/19/05-9/23/05
(3) Parts characterization/Measurement techniques
(3) Jenny Nguyen
(3) Publishing Group/Thoughts
(3) Beta-Galactosidase Assay (A better Miller)
(2) Students for Global Sustainability Wiki
(2) Pour YPD plates
(2) Lab meetings
(2) Synthetic Genomics Study
(1) Rune Linding
(1) AlexLabNotebook/10/1/05-10/7/05
(1) AlexLabNotebook/9/24/05-9/30/05
(1) Denaturing acrylamide gel purification of nucleic acids
(1) Linder Lab
(1) Andrew Hessel
(1) The BioBricks Foundation
(1) AlexLabNotebook/YeastRebuild/9/24/05-9/30/05
(1) AlexLabNotebook/YeastRebuild/10/1/05-10/7/05
(1) AlexLabNotebook/YeastRebuild/9/19/05-9/23/05
(1) Visualization
(1) Tabasco
(1) T7 Uncertainty
(1) Barry Canton
(1) Googlesearchheader
(1) Copyright
(1) How to publish a paper
(1) DNA sequence design to modulate evolutionary stability
(1) Counter-selectable markers
(1) Sriram Kosuri
(1) Registry Wish List
(1) Supergroup Mon Oct 17
(1) 2005 ICSB Conference
(1) AlexLabNotebook
(1) CcdB
(1) Vectors
(1) Parts Characterization
(1) Computing
(1) September 2005
(1) AlexLabNotebook/9/19/05-9/23/05

11/2005

Common: 744
Groups: 373
IGEM: 11
Protocols: 61
Materials: 14
Labs: 199

Details

Group Pages

(88) BE.109
(80) Synthetic Biology
(59) Sauer
(51) BE Board
(36) Silver
(25) Matsudaira
(12) Bell
(8) Suggs
(4) SfGS
(3) Bcanton
(2) Tregwiki
(2) Ellington
(2) 20.109(S07)
(1) Wikiomics

Lab Pages

IGEM Pages

(11) IGEM

Protocol Pages

(20) Endy:FACS calibration with beads
(9) Protocols
(6) Site-directed mutagenesis
(5) Methods to determine the size of an object in microns
(3) Phenol/chloroform extraction
(3) Ethanol precipitation of nucleic acids
(2) Knight:Micropure EZ and Microcon purification
(2) Knight:Colony PCR
(2) Endy:Victor3 plate reader
(2) Preparing chemically competent cells
(2) In vitro transcription with T7 RNA polymerase
(1) Assaying mating
(1) High Efficiency Transformation
(1) Order a Microfluidic Chip from the Foundry
(1) Resuspension of primers
(1) Endy:Pouring plates

Material Pages

Common Pages

12/2005

Common: 1094
Groups: 836
IGEM: 1
Protocols: 30
Materials: 19
Labs: 227

Details

Group Pages

(148) Lauffenburger
(134) BE Board
(98) Silver
(88) BE.109
(71) Sosnick
(68) Synthetic Biology
(53) Chandra
(53) University of Chicago
(23) Labs
(19) Pan
(17) Sauer
(10) DeLong
(8) Bbf
(8) Eduardo A Silva
(6) Eigencluster
(5) Suma
(5) MIT
(5) Dewikify
(3) Keasling
(2) Tregwiki
(2) Eduardo A. Silva
(2) Bcanton
(2) Rao
(2) KM
(2) Matsudaira
(1) Fhuang
(1) Wikiomics

Lab Pages

IGEM Pages

(1) IGEM

Protocol Pages

(9) Protocols
(7) MIT:Sequencing BioBrick DNA
(4) Keating:Experimental Protocols:HPLC
(3) Endy:Victor3 plate reader
(3) Sequencing DNA
(1) Dropout plates for yeast
(1) Electrocompetent cells
(1) PNK Treatment of DNA Ends
(1) Knight:Preparing electrocompetent cells

Material Pages

Common Pages

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