Oneill Lab:Enzymes: Difference between revisions
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(New page: '''Michael J. O'Neill Lab''' University of Connecticut Department of Molecular and Cell Biology ==Lab Stock Enzymes== Preparation of lab stock enzymes and buffers ===10x E...) |
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Aliquot and store at -20°C | Aliquot and store at -20°C | ||
==Links and Contacts== | |||
[[User:Seth|Seth Kasowitz]] [mailto:seth.kasowitz@uconn.edu] | |||
[http://www.oneill.mcb.uconn.edu/Michael/MONEILLhome.html|O'Neill Lab Homepage] | |||
Revision as of 13:10, 30 April 2007
Michael J. O'Neill Lab University of Connecticut Department of Molecular and Cell Biology
Lab Stock Enzymes
Preparation of lab stock enzymes and buffers
10x EcoRI Buffer
- Combine
- 8mL 2.5M Tris
- 2mL 5M NaCl
- 1mL 1M MgCl2
- 50μL TritonX-100
- Adjust pH to 7.5 with 12M HCl
- Add water to 20mL
Proteinase K
Makes 0.5mL at 20mg/ml
- 10μL 1M CaCl2
- 5μL 1M Tris pH 7.5
- 250μL Glycerol
- Pipette with cut tip
- Use under flame
- 10mg ProK
- Stored in 4°C dessicator
- 235μL H2O
Aliquot and store at -20°C
