OhioMod2013:Methods/Liposomes: Difference between revisions
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(New page: Got liposomes from Bryant Yung of Dr. Lee's lab. Two tubes of: *QTCN: Quaternary-Tertiary Lipid Amine Cationic Nanoparticles (1 mg/mL) *SPLN: Small Peptide Lipid Nanoparticles (2 mg/mL) Ke...) |
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Revision as of 06:38, 2 July 2013
Got liposomes from Bryant Yung of Dr. Lee's lab. Two tubes of:
- QTCN: Quaternary-Tertiary Lipid Amine Cationic Nanoparticles (1 mg/mL)
- SPLN: Small Peptide Lipid Nanoparticles (2 mg/mL)
Kept in 40% ethanol in 10 mM citric acid buffer (pH of 5). The lipid mixture is composed of DODAP, Lac-DOPE, DOPE, DMG-PEG, and with or without gramacidin A, at a molar ratio of 50:10:28:2:10 respectively.
Combine with DNA origami
Take lipid stock, combine with oligo at ratio of 1:15 DNA/lipid(weight/weight) in 10x transfection medium. Set aside 10-15 min to allow for electrostatic interaction. Dilute to final volume Extrude through polycarbonate filter, or other sterile filter in general
