ONPF

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==Details==
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*CAS No. 1154-94-5
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*C<sub>12</sub>H<sub>15</sub>NO<sub>7</sub>
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*Formula weight 285.25 g/mol
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*Not very soluble in water at room temperature
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==Notes==
Some early work on lacI generated various small molecule analogs similar in structure to IPTG in an effort to find more compounds which modulate the activity of lacI.  ONPF was found to stabilize lacI when bound to DNA.  Recent papers primarily used ONPF in structural determinations of the DNA bound form of lacI.
Some early work on lacI generated various small molecule analogs similar in structure to IPTG in an effort to find more compounds which modulate the activity of lacI.  ONPF was found to stabilize lacI when bound to DNA.  Recent papers primarily used ONPF in structural determinations of the DNA bound form of lacI.
Based on these early experiments, I decided to test whether ONPF could act as an anti-inducer in our flow measurements.  The fluorescence levels of lacI containing cells with plasmid-based Plac.YFP in the presence and absence of 1mM ONPF were compared using [[MIT Flow Cytometer Core Facility | Flow Cytometry]].  However, no difference in fluorescence was observed.
Based on these early experiments, I decided to test whether ONPF could act as an anti-inducer in our flow measurements.  The fluorescence levels of lacI containing cells with plasmid-based Plac.YFP in the presence and absence of 1mM ONPF were compared using [[MIT Flow Cytometer Core Facility | Flow Cytometry]].  However, no difference in fluorescence was observed.

Revision as of 12:25, 8 June 2005

Details

  • CAS No. 1154-94-5
  • C12H15NO7
  • Formula weight 285.25 g/mol
  • Not very soluble in water at room temperature

Notes

Some early work on lacI generated various small molecule analogs similar in structure to IPTG in an effort to find more compounds which modulate the activity of lacI. ONPF was found to stabilize lacI when bound to DNA. Recent papers primarily used ONPF in structural determinations of the DNA bound form of lacI.

Based on these early experiments, I decided to test whether ONPF could act as an anti-inducer in our flow measurements. The fluorescence levels of lacI containing cells with plasmid-based Plac.YFP in the presence and absence of 1mM ONPF were compared using Flow Cytometry. However, no difference in fluorescence was observed.

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