ONP: Difference between revisions
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*[[Beta-galactosidase]] cleaves [[ONPG]] to produce ONP and galactose. | *[[Beta-galactosidase]] cleaves [[ONPG]] to produce ONP and galactose. | ||
*The molar extinction coefficient is reported to be either 4500 M<sup>-1</sup>cm<sup>-1</sup> <cite>Skoneczny-BiochemJ-2000</cite> or 4900 M<sup>-1</sup>cm<sup>-1</sup> <cite>Onishi-ApplEnvironMicrobiol-1995</cite> (not original references, better references would be preferable). | *The molar extinction coefficient is reported to be either 4500 M<sup>-1</sup>cm<sup>-1</sup> <cite>Skoneczny-BiochemJ-2000</cite> or 4900 M<sup>-1</sup>cm<sup>-1</sup> <cite>Onishi-ApplEnvironMicrobiol-1995</cite> (not original references, better references would be preferable). | ||
*Slightly soluble in water (0.21 g/100ml or 2.1mg/mL) | |||
==References== | ==References== | ||
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#Skoneczny-BiochemJ-2000 pmid=10926859 | #Skoneczny-BiochemJ-2000 pmid=10926859 | ||
#Onishi-ApplEnvironMicrobiol-1995 pmid=8526517 | #Onishi-ApplEnvironMicrobiol-1995 pmid=8526517 | ||
#o-nitrophenol [http://chemicalland21.com/arokorhi/industrialchem/organic/o-NITROPHENOL.htm Chemical information] | |||
</biblio> | </biblio> | ||
[[Category:Material]] [[Category:Chemical]] | [[Category:Material]] [[Category:Chemical]] | ||
Latest revision as of 08:53, 30 October 2007
o-nitrophenol
- Beta-galactosidase cleaves ONPG to produce ONP and galactose.
- The molar extinction coefficient is reported to be either 4500 M-1cm-1 [1] or 4900 M-1cm-1 [2] (not original references, better references would be preferable).
- Slightly soluble in water (0.21 g/100ml or 2.1mg/mL)
References
- Skoneczny M and Rytka J. Oxygen and haem regulate the synthesis of peroxisomal proteins: catalase A, acyl-CoA oxidase and Pex1p in the yeast Saccharomyces cerevisiae; the regulation of these proteins by oxygen is not mediated by haem. Biochem J. 2000 Aug 15;350 Pt 1(Pt 1):313-9.
- Onishi N and Tanaka T. Purification and properties of a novel thermostable galacto-oligosaccharide-producing beta-galactosidase from Sterigmatomyces elviae CBS8119. Appl Environ Microbiol. 1995 Nov;61(11):4026-30. DOI:10.1128/aem.61.11.4026-4030.1995 |
