Northern blot: Difference between revisions
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== External links == | == External links == | ||
* [http://www.protocol-online.org/cgi-bin/htsearch?config=htdig_pol&restrict=&exclude=&words=northern+blot archived protocol conversations from Protocol Online] | * [[Image:2stars.png]] [http://www.protocol-online.org/cgi-bin/htsearch?config=htdig_pol&restrict=&exclude=&words=northern+blot archived protocol conversations from Protocol Online] | ||
* [http://www.protocol-online.org/prot/Molecular_Biology/RNA/Northern_Blotting/ index of RNA blot protocols from Protocol Online] | * [[Image:2stars.png]] [http://www.protocol-online.org/prot/Molecular_Biology/RNA/Northern_Blotting/ index of RNA blot protocols from Protocol Online] | ||
* [http://kellylab.wustl.edu/protocols/RNA_and_northern_blot3-3-2006.pdf RNA preparation and blotting protocol] (2006) by Kelly lab, Washington Uni | * [[Image:2stars.png]] [http://kellylab.wustl.edu/protocols/RNA_and_northern_blot3-3-2006.pdf RNA preparation and blotting protocol] (2006) by Kelly lab, Washington Uni | ||
* [http://biology.semo.edu/agathman/research/protocols/northern.htm RNA blot protocol] by Allen Gathman from Southeast Missouri State Uni | * [[Image:2stars.png]] [http://biology.semo.edu/agathman/research/protocols/northern.htm RNA blot protocol] by Allen Gathman from Southeast Missouri State Uni | ||
* [http://www.ambion.com/techlib/tn/63/631.html Techniques to detect mRNA - includes RNA blot], Ambion TechNotes | * [[Image:1stars.png]] [http://www.ambion.com/techlib/tn/63/631.html Techniques to detect mRNA - includes RNA blot], Ambion TechNotes | ||
* [http://www.roche-applied-science.com/pack-insert/2039672a.pdf Pack insert DIG RNA detection kit], Roche | * [[Image:1stars.png]] [http://www.roche-applied-science.com/pack-insert/2039672a.pdf Pack insert DIG RNA detection kit], Roche | ||
Revision as of 07:05, 13 March 2008
The RNA blot or Northern blot (named after the Southern blot for DNA fragments) is a molecular biology technique used to separate and identify pieces of RNA. RNA molecules are separated by mass on a gel, transferred (blotted) onto a cellulose or nylon membrane, and then labelled with complementary DNA or RNA molecules. These probes are either radioactive, typically 32P, or contain labelled nucleotides, e.g. DIG-dNTPs, recognisable by antibodies. RNA molecules can be detected and roughly quantified via probe hybridisation.
See also
- RNA
- RNA Extraction
- RNA electrophoresis
- Endy:Northern Blot, 32P End-Labeled Probes
- BE.109:Systems engineering/Measuring DNA, RNA, protein
External links
archived protocol conversations from Protocol Online- index of RNA blot protocols from Protocol Online
- RNA preparation and blotting protocol (2006) by Kelly lab, Washington Uni
- RNA blot protocol by Allen Gathman from Southeast Missouri State Uni
Techniques to detect mRNA - includes RNA blot, Ambion TechNotes- Pack insert DIG RNA detection kit, Roche
