Northern blot: Difference between revisions
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== See also == | == See also == | ||
* [[ | * [[RNA]] | ||
* [[RNA Extraction]] | |||
* [[RNA electrophoresis]] | * [[RNA electrophoresis]] | ||
* [[Endy:Northern Blot, 32P End-Labeled Probes]] | * [[Endy:Northern Blot, 32P End-Labeled Probes]] | ||
* [[RNA | * [[BE.109:Systems engineering/Measuring DNA, RNA, protein]] | ||
Revision as of 06:43, 13 March 2008
The RNA blot or Northern blot (named after the Southern blot for DNA fragments) is a molecular biology technique used to separate and identify pieces of RNA. RNA molecules are separated by mass on a gel, transferred (blotted) onto a cellulose or nylon membrane, and then labelled with complementary DNA or RNA molecules. These probes are either radioactive, typically 32P, or contain labelled nucleotides, e.g. DIG-dNTPs, recognisable by antibodies. RNA molecules can be detected and roughly quantified via probe hybridisation.