Nissl staining

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The Nissl staining is a dye combination mostly staining RNA in dark blue to purple.

Principle

The Nissl staining employs a mixture of basic dyes (aniline, thionine, or cresyl violet) which stains negatively charged RNA.

Target of the dye

Nissl staining typically marks the ER due to ribosomal RNA as well as the nucleus.

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