http://openwetware.org/index.php?title=Nissl_staining&feed=atom&action=history 2013-05-24T23:29:50Z Revision history for this page on the wiki MediaWiki 1.13.2 http://openwetware.org/index.php?title=Nissl_staining&diff=416266&oldid=prev 2010-05-20T12:48:08Z

<p>external links</p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 12:48, 20 May 2010</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 16:</td> <td colspan="2" class="diff-lineno">Line 16:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* [http://www.psy.jhu.edu/~fortune/Nissl.html Nissl staining protocol from the Fortune lab, Johns Hopkins]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* [http://www.psy.jhu.edu/~fortune/Nissl.html Nissl staining protocol from the Fortune lab, Johns Hopkins]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* [http://psych.colorado.edu/~dbarth/PDFs/4052/4052%20Manual%20Chapters/Histology%20I.pdf Nissl staining protocol from Barth course material, U of Colorado at Boulder]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* [http://psych.colorado.edu/~dbarth/PDFs/4052/4052%20Manual%20Chapters/Histology%20I.pdf Nissl staining protocol from Barth course material, U of Colorado at Boulder]</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* [http://www.neuralstainkit.com/index.php?pr=Ready-to-Use_Staining_Solutions Commercial Nissl staining solutions w photos of stained sections]&nbsp; </ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category:Material]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category:Material]]</div></td></tr> <!-- diff generator: internal 2013-05-24 23:29:50 --> </table>

Jakob Suckale http://openwetware.org/index.php?title=Nissl_staining&diff=416265&oldid=prev 2010-05-20T12:44:57Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 12:44, 20 May 2010</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Journal.pone.0008762.s003.B Nissl stain.jpg|thumb|right|Nissl staining (blue) in the substantia nigra pars compacta of mice from (Pan-Montojo 2010)]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Journal.pone.0008762.s003.B Nissl stain.jpg|thumb|right|Nissl staining (blue) in the substantia nigra pars compacta of mice from (Pan-Montojo 2010)]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The '''Nissl staining''' is a classic nucleic acid staining method traditionally used on nervous tissue sections. The active dye in the staining solution can vary, but toluidine blue or cresyl violet are common components. &nbsp;</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The '''Nissl staining''' is a classic nucleic acid staining method traditionally used on nervous tissue sections. The active dye in the staining solution can vary, but toluidine blue or cresyl violet are common components<ins class="diffchange diffchange-inline">. Nissl is also an outdated term for the ER</ins>.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Principle ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Principle ==</div></td></tr> <tr><td colspan="2" class="diff-lineno">Line 15:</td> <td colspan="2" class="diff-lineno">Line 15:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* [http://faculty.washington.edu/rhevner/Nissl.doc Nissl staining of paraffin section protocol from the Hevner lab U Washington]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* [http://faculty.washington.edu/rhevner/Nissl.doc Nissl staining of paraffin section protocol from the Hevner lab U Washington]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* [http://www.psy.jhu.edu/~fortune/Nissl.html Nissl staining protocol from the Fortune lab, Johns Hopkins]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* [http://www.psy.jhu.edu/~fortune/Nissl.html Nissl staining protocol from the Fortune lab, Johns Hopkins]</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* [http://psych.colorado.edu/~dbarth/PDFs/4052/4052%20Manual%20Chapters/Histology%20I.pdf Nissl staining protocol from Barth course material, U of Colorado at Boulder]</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category:Material]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category:Material]]</div></td></tr> <!-- diff generator: internal 2013-05-24 23:29:50 --> </table>

Jakob Suckale http://openwetware.org/index.php?title=Nissl_staining&diff=416264&oldid=prev 2010-05-20T12:41:39Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 12:41, 20 May 2010</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Journal.pone.0008762.s003.B Nissl stain.jpg|thumb|right|Nissl staining (blue) in the substantia nigra pars compacta of mice from (Pan-Montojo 2010)]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Journal.pone.0008762.s003.B Nissl stain.jpg|thumb|right|Nissl staining (blue) in the substantia nigra pars compacta of mice from (Pan-Montojo 2010)]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The '''Nissl staining''' is a classic <del class="diffchange diffchange-inline">dye combination mostly binding to </del>nucleic acid <del class="diffchange diffchange-inline">and </del>staining <del class="diffchange diffchange-inline">it in dark blue to purple</del>. The <del class="diffchange diffchange-inline">Nissl </del>staining <del class="diffchange diffchange-inline">is most well known for its use in nervous tissue histology</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The '''Nissl staining''' is a classic nucleic acid staining <ins class="diffchange diffchange-inline">method traditionally used on nervous tissue sections</ins>. The <ins class="diffchange diffchange-inline">active dye in the </ins>staining <ins class="diffchange diffchange-inline">solution can vary, but toluidine blue or cresyl violet are common components</ins>. &nbsp;</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Principle ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Principle ==</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">The Nissl staining employs a mixture of </del>basic <del class="diffchange diffchange-inline">dyes </del>(aniline, thionine, or cresyl violet) <del class="diffchange diffchange-inline">which stains </del>negatively charged RNA.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">A </ins>basic <ins class="diffchange diffchange-inline">dye </ins>(aniline, thionine, or cresyl violet) <ins class="diffchange diffchange-inline">binds to </ins>negatively charged <ins class="diffchange diffchange-inline">nucleic acids like </ins>RNA <ins class="diffchange diffchange-inline">and DNA</ins>.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Target of the dye ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Target of the dye ==</div></td></tr> <tr><td colspan="2" class="diff-lineno">Line 13:</td> <td colspan="2" class="diff-lineno">Line 13:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== External links ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== External links ==</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* [http://en.wikipedia.org/wiki/Nissl_staining#Named_histology_concepts Nissl staining section from the biography of the eponymous scientist Franz Nissl in the Wikipedia]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* [http://en.wikipedia.org/wiki/Nissl_staining#Named_histology_concepts Nissl staining section from the biography of the eponymous scientist Franz Nissl in the Wikipedia]</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* [http://faculty.washington.edu/rhevner/Nissl.doc Nissl staining of paraffin section protocol from the Hevner <del class="diffchange diffchange-inline">Lab </del>U Washington]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* [http://faculty.washington.edu/rhevner/Nissl.doc Nissl staining of paraffin section protocol from the Hevner <ins class="diffchange diffchange-inline">lab </ins>U Washington<ins class="diffchange diffchange-inline">]</ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* [http://www.psy.jhu.edu/~fortune/Nissl.html Nissl staining protocol from the Fortune lab, Johns Hopkins</ins>]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category:Material]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Category:Material]]</div></td></tr> <!-- diff generator: internal 2013-05-24 23:29:50 --> </table>

Jakob Suckale http://openwetware.org/index.php?title=Nissl_staining&diff=416256&oldid=prev 2010-05-20T12:06:13Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 12:06, 20 May 2010</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Journal.pone.0008762.s003.B Nissl stain.jpg|thumb|right|Nissl staining in the substantia nigra pars compacta of mice from (Pan-Montojo 2010)]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Journal.pone.0008762.s003.B Nissl stain.jpg|thumb|right|Nissl staining <ins class="diffchange diffchange-inline">(blue) </ins>in the substantia nigra pars compacta of mice from (Pan-Montojo 2010)]]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The '''Nissl staining''' is a classic dye combination mostly binding to nucleic acid and staining it in dark blue to purple. The Nissl staining is most well known for its use in nervous tissue histology.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The '''Nissl staining''' is a classic dye combination mostly binding to nucleic acid and staining it in dark blue to purple. The Nissl staining is most well known for its use in nervous tissue histology.</div></td></tr> <!-- diff generator: internal 2013-05-24 23:29:50 --> </table>

Jakob Suckale http://openwetware.org/index.php?title=Nissl_staining&diff=416255&oldid=prev 2010-05-20T11:47:14Z

<p>categories</p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 11:47, 20 May 2010</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 14:</td> <td colspan="2" class="diff-lineno">Line 14:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* [http://en.wikipedia.org/wiki/Nissl_staining#Named_histology_concepts Nissl staining section from the biography of the eponymous scientist Franz Nissl in the Wikipedia]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* [http://en.wikipedia.org/wiki/Nissl_staining#Named_histology_concepts Nissl staining section from the biography of the eponymous scientist Franz Nissl in the Wikipedia]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* [http://faculty.washington.edu/rhevner/Nissl.doc Nissl staining of paraffin section protocol from the Hevner Lab U Washington]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* [http://faculty.washington.edu/rhevner/Nissl.doc Nissl staining of paraffin section protocol from the Hevner Lab U Washington]</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Category:Material]]</ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Category:Protocol]]</ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Category:Microscopy]]</ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Category:Dye]]</ins></div></td></tr> <!-- diff generator: internal 2013-05-24 23:29:50 --> </table>

Jakob Suckale http://openwetware.org/index.php?title=Nissl_staining&diff=416254&oldid=prev 2010-05-20T11:46:22Z

<p><span class="autocomment">Target of the dye</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 11:46, 20 May 2010</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 9:</td> <td colspan="2" class="diff-lineno">Line 9:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Target of the dye ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Target of the dye ==</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Nissl staining typically marks the ER due to ribosomal RNA as well as the nucleus.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Nissl staining typically marks the ER due to ribosomal RNA as well as the nucleus <ins class="diffchange diffchange-inline">and other accumulations of nucleic acid</ins>.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== External links ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== External links ==</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* [http://en.wikipedia.org/wiki/Nissl_staining#Named_histology_concepts Nissl staining section from the biography of the eponymous scientist Franz Nissl in the Wikipedia]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* [http://en.wikipedia.org/wiki/Nissl_staining#Named_histology_concepts Nissl staining section from the biography of the eponymous scientist Franz Nissl in the Wikipedia]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* [http://faculty.washington.edu/rhevner/Nissl.doc Nissl staining of paraffin section protocol from the Hevner Lab U Washington]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* [http://faculty.washington.edu/rhevner/Nissl.doc Nissl staining of paraffin section protocol from the Hevner Lab U Washington]</div></td></tr> <!-- diff generator: internal 2013-05-24 23:29:50 --> </table>

Jakob Suckale http://openwetware.org/index.php?title=Nissl_staining&diff=416248&oldid=prev 2010-05-20T10:36:02Z

<p>illustration</p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 10:36, 20 May 2010</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Image:Journal.pone.0008762.s003.B Nissl stain.jpg|thumb|right|Nissl staining in the substantia nigra pars compacta of mice from (Pan-Montojo 2010)]]</ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The '''Nissl staining''' is a classic dye combination mostly binding to nucleic acid and staining it in dark blue to purple. The Nissl staining is most well known for its use in nervous tissue histology.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The '''Nissl staining''' is a classic dye combination mostly binding to nucleic acid and staining it in dark blue to purple. The Nissl staining is most well known for its use in nervous tissue histology.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <!-- diff generator: internal 2013-05-24 23:29:50 --> </table>

Jakob Suckale http://openwetware.org/index.php?title=Nissl_staining&diff=416246&oldid=prev 2010-05-20T10:31:37Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 10:31, 20 May 2010</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The '''Nissl staining''' is a dye combination mostly staining <del class="diffchange diffchange-inline">RNA </del>in dark blue to purple.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The '''Nissl staining''' is a <ins class="diffchange diffchange-inline">classic </ins>dye combination mostly <ins class="diffchange diffchange-inline">binding to nucleic acid and </ins>staining <ins class="diffchange diffchange-inline">it </ins>in dark blue to purple<ins class="diffchange diffchange-inline">. The Nissl staining is most well known for its use in nervous tissue histology</ins>.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Principle ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Principle ==</div></td></tr> <!-- diff generator: internal 2013-05-24 23:29:50 --> </table>

Jakob Suckale http://openwetware.org/index.php?title=Nissl_staining&diff=416243&oldid=prev 2010-05-20T10:18:36Z

<p><span class="autocomment">Target of the dye</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 10:18, 20 May 2010</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 6:</td> <td colspan="2" class="diff-lineno">Line 6:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Target of the dye ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Target of the dye ==</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Nissl staining typically marks the ER due to ribosomal RNA as well as the nucleus.</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== External links ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== External links ==</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* [http://en.wikipedia.org/wiki/Nissl_staining#Named_histology_concepts Nissl staining section from the biography of the eponymous scientist Franz Nissl in the Wikipedia]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* [http://en.wikipedia.org/wiki/Nissl_staining#Named_histology_concepts Nissl staining section from the biography of the eponymous scientist Franz Nissl in the Wikipedia]</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* [http://faculty.washington.edu/rhevner/Nissl.doc Nissl staining of paraffin section protocol from the Hevner Lab U Washington]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* [http://faculty.washington.edu/rhevner/Nissl.doc Nissl staining of paraffin section protocol from the Hevner Lab U Washington]</div></td></tr> <!-- diff generator: internal 2013-05-24 23:29:50 --> </table>

Jakob Suckale http://openwetware.org/index.php?title=Nissl_staining&diff=416242&oldid=prev 2010-05-20T10:16:28Z

<p><span class="autocomment">Principle</span></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 10:16, 20 May 2010</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 2:</td> <td colspan="2" class="diff-lineno">Line 2:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Principle ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Principle ==</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">The Nissl staining employs a mixture of basic dyes (aniline, thionine, or cresyl violet) which stains negatively charged RNA.</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Target of the dye ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Target of the dye ==</div></td></tr> <!-- diff generator: internal 2013-05-24 23:29:50 --> </table>

Jakob Suckale