Nissl staining

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The '''Nissl staining''' is a classic dye combination mostly binding to nucleic acid and staining it in dark blue to purple. The Nissl staining is most well known for its use in nervous tissue histology.
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[[Image:Journal.pone.0008762.s003.B Nissl stain.jpg|thumb|right|Nissl staining (blue) in the substantia nigra pars compacta of mice from (Pan-Montojo 2010)]]
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The '''Nissl staining''' is a classic nucleic acid staining method traditionally used on nervous tissue sections. The active dye in the staining solution can vary, but toluidine blue or cresyl violet are common components. Nissl is also an outdated term for the ER.
== Principle ==
== Principle ==
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The Nissl staining employs a mixture of basic dyes (aniline, thionine, or cresyl violet) which stains negatively charged RNA.
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A basic dye (aniline, thionine, or cresyl violet) binds to negatively charged nucleic acids like RNA and DNA.
== Target of the dye ==
== Target of the dye ==
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Nissl staining typically marks the ER due to ribosomal RNA as well as the nucleus.
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Nissl staining typically marks the ER due to ribosomal RNA as well as the nucleus and other accumulations of nucleic acid.
== External links ==
== External links ==
* [http://en.wikipedia.org/wiki/Nissl_staining#Named_histology_concepts Nissl staining section from the biography of the eponymous scientist Franz Nissl in the Wikipedia]
* [http://en.wikipedia.org/wiki/Nissl_staining#Named_histology_concepts Nissl staining section from the biography of the eponymous scientist Franz Nissl in the Wikipedia]
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* [http://faculty.washington.edu/rhevner/Nissl.doc Nissl staining of paraffin section protocol from the Hevner Lab U Washington]
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* [http://faculty.washington.edu/rhevner/Nissl.doc Nissl staining of paraffin section protocol from the Hevner lab U Washington]
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* [http://www.psy.jhu.edu/~fortune/Nissl.html Nissl staining protocol from the Fortune lab, Johns Hopkins]
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* [http://psych.colorado.edu/~dbarth/PDFs/4052/4052%20Manual%20Chapters/Histology%20I.pdf Nissl staining protocol from Barth course material, U of Colorado at Boulder]
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* [http://www.neuralstainkit.com/index.php?pr=Ready-to-Use_Staining_Solutions Commercial Nissl staining solutions w photos of stained sections] 
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[[Category:Material]]
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[[Category:Protocol]]
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[[Category:Microscopy]]
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[[Category:Dye]]

Current revision

Nissl staining (blue) in the substantia nigra pars compacta of mice from (Pan-Montojo 2010)
Nissl staining (blue) in the substantia nigra pars compacta of mice from (Pan-Montojo 2010)

The Nissl staining is a classic nucleic acid staining method traditionally used on nervous tissue sections. The active dye in the staining solution can vary, but toluidine blue or cresyl violet are common components. Nissl is also an outdated term for the ER.

Principle

A basic dye (aniline, thionine, or cresyl violet) binds to negatively charged nucleic acids like RNA and DNA.

Target of the dye

Nissl staining typically marks the ER due to ribosomal RNA as well as the nucleus and other accumulations of nucleic acid.

External links

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