Nissl staining

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(illustration)
(Target of the dye)
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== Target of the dye ==
== Target of the dye ==
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Nissl staining typically marks the ER due to ribosomal RNA as well as the nucleus.
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Nissl staining typically marks the ER due to ribosomal RNA as well as the nucleus and other accumulations of nucleic acid.
== External links ==
== External links ==
* [http://en.wikipedia.org/wiki/Nissl_staining#Named_histology_concepts Nissl staining section from the biography of the eponymous scientist Franz Nissl in the Wikipedia]
* [http://en.wikipedia.org/wiki/Nissl_staining#Named_histology_concepts Nissl staining section from the biography of the eponymous scientist Franz Nissl in the Wikipedia]
* [http://faculty.washington.edu/rhevner/Nissl.doc Nissl staining of paraffin section protocol from the Hevner Lab U Washington]
* [http://faculty.washington.edu/rhevner/Nissl.doc Nissl staining of paraffin section protocol from the Hevner Lab U Washington]

Revision as of 06:46, 20 May 2010

Nissl staining in the substantia nigra pars compacta of mice from (Pan-Montojo 2010)
Nissl staining in the substantia nigra pars compacta of mice from (Pan-Montojo 2010)

The Nissl staining is a classic dye combination mostly binding to nucleic acid and staining it in dark blue to purple. The Nissl staining is most well known for its use in nervous tissue histology.

Principle

The Nissl staining employs a mixture of basic dyes (aniline, thionine, or cresyl violet) which stains negatively charged RNA.

Target of the dye

Nissl staining typically marks the ER due to ribosomal RNA as well as the nucleus and other accumulations of nucleic acid.

External links

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