Nissl staining

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The '''Nissl staining''' is a dye combination mostly staining RNA in dark blue to purple.
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The '''Nissl staining''' is a classic dye combination mostly binding to nucleic acid and staining it in dark blue to purple. The Nissl staining is most well known for its use in nervous tissue histology.
== Principle ==
== Principle ==

Revision as of 06:31, 20 May 2010

The Nissl staining is a classic dye combination mostly binding to nucleic acid and staining it in dark blue to purple. The Nissl staining is most well known for its use in nervous tissue histology.

Principle

The Nissl staining employs a mixture of basic dyes (aniline, thionine, or cresyl violet) which stains negatively charged RNA.

Target of the dye

Nissl staining typically marks the ER due to ribosomal RNA as well as the nucleus.

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