Neomycin

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==Experimental==
==Experimental==
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'''target organism''': bacteria, mammalian cells
+
*'''target organism''': bacteria, mammalian cells
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'''stock solution''': 10mg/ml in water
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*'''stock solution''': 10mg/ml in water
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'''working solution''': 10-50µg/ml (stable at RT for 5days)
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*'''working solution''': 10-50µg/ml (stable at RT for 5days)
==Description==
==Description==
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'''mode of action''': binds to ribosomal components and inhibits protein synthesis.
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*'''mode of action''': binds to ribosomal components and inhibits protein synthesis.
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'''mode of resistance''': ''neo/kan'' resistance gene is an aminoglycoside 3'-phosphotransferase, which inactivates [[G418]], neomycin, and [[kanamycin]] by phosphorylation.
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*'''mode of resistance''': ''neo/kan'' resistance gene is an aminoglycoside 3'-phosphotransferase, which inactivates [[G418]], neomycin, and [[kanamycin]] by phosphorylation.
==Links==
==Links==

Revision as of 09:07, 4 August 2006

Experimental

  • target organism: bacteria, mammalian cells
  • stock solution: 10mg/ml in water
  • working solution: 10-50µg/ml (stable at RT for 5days)

Description

  • mode of action: binds to ribosomal components and inhibits protein synthesis.
  • mode of resistance: neo/kan resistance gene is an aminoglycoside 3'-phosphotransferase, which inactivates G418, neomycin, and kanamycin by phosphorylation.

Links

  • related antibiotics: kanamycin, G418
  • list of cell culture reagents by Krackeler [1]
  • list of cell culture reagents by USB [2]
  • forum thread on difference between neomycin & G418 [3]
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