Neomycin
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==Experimental== | ==Experimental== | ||
| - | + | '''target organism''': bacteria, mammalian cells | |
| - | bacteria, mammalian cells | + | '''stock solution''': 10mg/ml in water |
| - | + | '''working solution''': 10-50µg/ml (stable at RT for 5days) | |
| - | + | ||
| - | 10mg/ml in water | + | |
| - | + | ||
| - | + | ||
| - | 10-50µg/ml | + | |
==Description== | ==Description== | ||
| - | + | '''mode of action''': binds to ribosomal components and inhibits protein synthesis. | |
| - | + | '''mode of resistance''': ''neo/kan'' resistance gene is an aminoglycoside 3'-phosphotransferase, which inactivates [[G418]], neomycin, and [[kanamycin]] by phosphorylation. | |
| - | + | ||
| - | + | ||
| - | ''neo/kan'' resistance gene is an aminoglycoside 3'-phosphotransferase, which inactivates [[G418]], neomycin, and [[kanamycin]] by phosphorylation. | + | |
| - | == | + | ==Links== |
| - | + | ||
| - | + | * related antibiotics: [[kanamycin]], [[G418]] | |
* list of cell culture reagents by Krackeler [http://www.krackeler.com/products/fid/2910] | * list of cell culture reagents by Krackeler [http://www.krackeler.com/products/fid/2910] | ||
* list of cell culture reagents by USB [http://www.usbweb.com/reference2.asp?id_ref=14] | * list of cell culture reagents by USB [http://www.usbweb.com/reference2.asp?id_ref=14] | ||
* forum thread on difference between neomycin & G418 [http://www.protocol-online.org/forums/index.php?showtopic=10608&hl=neomycin] | * forum thread on difference between neomycin & G418 [http://www.protocol-online.org/forums/index.php?showtopic=10608&hl=neomycin] | ||
Revision as of 09:06, 4 August 2006
Experimental
target organism: bacteria, mammalian cells stock solution: 10mg/ml in water working solution: 10-50µg/ml (stable at RT for 5days)
Description
mode of action: binds to ribosomal components and inhibits protein synthesis. mode of resistance: neo/kan resistance gene is an aminoglycoside 3'-phosphotransferase, which inactivates G418, neomycin, and kanamycin by phosphorylation.
