Neomycin
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==Experimental== | ==Experimental== | ||
| - | + | *'''target organism''': bacteria, mammalian cells | |
| - | + | *'''stock solution''': 10mg/ml in water | |
| + | *'''working solution''': 10-50µg/ml (stable at RT for 5days) | ||
| - | == | + | ==Description== |
| + | |||
| + | *'''mode of action''': binds to ribosomal components and inhibits protein synthesis. | ||
| + | *'''mode of resistance''': ''neo/kan'' resistance gene is an aminoglycoside 3'-phosphotransferase, which inactivates [[G418]], neomycin, and [[kanamycin]] by phosphorylation. | ||
| + | |||
| + | ==Links== | ||
| + | |||
| + | * related antibiotics: [[kanamycin]], [[G418]] | ||
| + | * list of cell culture reagents by Krackeler [http://www.krackeler.com/products/fid/2910] | ||
| + | * list of cell culture reagents by USB [http://www.usbweb.com/reference2.asp?id_ref=14] | ||
| + | * forum thread on difference between neomycin & G418 [http://www.protocol-online.org/forums/index.php?showtopic=10608&hl=neomycin] | ||
| + | |||
| + | [[Category:Material]] [[Category:Antibiotic]] | ||
Current revision
Experimental
- target organism: bacteria, mammalian cells
- stock solution: 10mg/ml in water
- working solution: 10-50µg/ml (stable at RT for 5days)
Description
- mode of action: binds to ribosomal components and inhibits protein synthesis.
- mode of resistance: neo/kan resistance gene is an aminoglycoside 3'-phosphotransferase, which inactivates G418, neomycin, and kanamycin by phosphorylation.
