Nelson:To Do - Sid: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
m (→The List) |
m (→The List) |
||
| Line 12: | Line 12: | ||
</div> | </div> | ||
==The List== | ==The List== | ||
*prepare | *prepare CaCl<sub>2</sub> competent Rosetta pLys cells | ||
*transform RNAp into freshly made competent cells | *transform RNAp into freshly made competent cells | ||
*spin down the 6L of cells found in cold room | *spin down the 6L of cells found in cold room | ||
Revision as of 06:31, 7 April 2009
The List
- prepare CaCl2 competent Rosetta pLys cells
- transform RNAp into freshly made competent cells
- spin down the 6L of cells found in cold room
- prepare DEAE cellulose according to instructions (look for it on protocols page)
- make tet-LB plates
- streak Xl1-blue onto tet-LB plate
- prepare top-agar
