Nelson:To Do - Sid: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
m (→The List) |
mNo edit summary |
||
| Line 13: | Line 13: | ||
==The List== | ==The List== | ||
*prepare DEAE cellulose according to instructions (look for it on protocols page) | |||
*rinse centrifuge bottles well and spin down the cells in the 4192 shaker, place in plastic bag, weigh and freeze | *rinse centrifuge bottles well and spin down the cells in the 4192 shaker, place in plastic bag, weigh and freeze | ||
*pour 2 SDS-PAGE gels, 12% | *pour 2 SDS-PAGE gels, 12% | ||
*make 1 L of 1 M Tris-HCl, pH 8.0 | *make 1 L of 1 M Tris-HCl, pH 8.0 | ||
*put away dry dishes | *put away dry dishes | ||
Revision as of 07:38, 2 April 2009
The List
- prepare DEAE cellulose according to instructions (look for it on protocols page)
- rinse centrifuge bottles well and spin down the cells in the 4192 shaker, place in plastic bag, weigh and freeze
- pour 2 SDS-PAGE gels, 12%
- make 1 L of 1 M Tris-HCl, pH 8.0
- put away dry dishes
