Nelson:Protocols: Difference between revisions
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Rubidium Chloride competent cells [[Wiese_Lab:Competent_Cell_Prep]]<br> | Rubidium Chloride competent cells [[Wiese_Lab:Competent_Cell_Prep]]<br> | ||
[http://ivaan.com/protocols/135.html Calcium Chloride competent cells ]<br> | [http://ivaan.com/protocols/135.html Calcium Chloride competent cells ]<br> | ||
[http://ivaan.com/protocols/133.html transformation of chemically competent E. coli]<br> | |||
Qiagen Miniprep [[Minipreps]]<br> | Qiagen Miniprep [[Minipreps]]<br> | ||
[[DNA_Precipitation]]<br> | [[DNA_Precipitation]]<br> | ||
Revision as of 06:29, 7 April 2009
Recipes
LB
M9 medium/minimal
TBE
SOB
SOC
10 X SDS-PAGE running buffer
50 X TAE buffer
Phosphate_buffer
Protocols
Rubidium Chloride competent cells Wiese_Lab:Competent_Cell_Prep
Calcium Chloride competent cells
transformation of chemically competent E. coli
Qiagen Miniprep Minipreps
DNA_Precipitation
DNA_ligation
Quickchange Mutagenesis Knight:Site-directed_mutagenesis/Single_site
preparing DEAE-cellulose
